| 胡玲 殷亮 吴畏 蔡琳 巩固.类缺血等多种预处理上调脂多糖应答基因在神经元中的表达[J].现代生物医学进展英文版,2014,14(13):2453-2456. |
| 类缺血等多种预处理上调脂多糖应答基因在神经元中的表达 |
| Ischemia-like and Various Preconditioning Promote the Expression ofLipopolysaccharide Response Gene in Neurons |
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| DOI： |
| 中文关键词: 预处理 LRG基因 脑缺血/ 再灌注 |
| 英文关键词: Precondition LRG gene Brain ischemic reperfusion |
| 基金项目: |
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| 中文摘要: |
| 目的：研究体外和体内多种预处理对小鼠神经元细胞内脂多糖应答基因(LRG)表达的影响。方法：利用qRT-PCR 和western
blot方法检测体外多种预处理(类缺血、高压氧、异氟和脂多糖) 对小鼠神经元细胞中LRG的转录水平和蛋白表达水平的影响；运
用小RNA 干扰方法(siRNA)沉默神经元细胞中LRG的表达，噻唑蓝(MTT)方法检测神经元细胞的生长情况；运用大脑中动脉阻
闭法(MCAO)建立多种预处理诱导脑缺血耐受的小鼠试验模型，qRT-PCR 和western blot 方法检测小鼠脑组织中LRG 转录水平
和蛋白水平的变化。结果：体外多种预处理明显上调LRG 在小鼠脑海马神经元细胞中的表达；沉默LRG在神经元中的表达明显
加快类缺血处理细胞的死亡；多种预处理建立的脑缺血耐受小鼠的脑组织中LRG 转录水平和蛋白表达水平明显升高。结论：类缺
血等多种预处理可上调神经元细胞内LRG的表达，但其在脑缺血再灌注损伤中的作用机制尚不清楚，需进一步的研究。 |
| 英文摘要: |
| Objective:To study the effect of various preconditioning on lipopolysaccharide response gene (LRG) expression in
neurons of mice in vitro and in vivo.Methods:LRG expression was silenced by small interference RNA (siRNA) and cell growth was
detected by MTT assay. The mouse model of ischemia tolerance under various pre-conditions was established by middle cerebral artery
occlusion (MCAO) and the transcription level and protein level of LRG in mouse brain tissues were determined by qRT-PCR and western
blot, respectively.Results:The mRNA and protein levels of LRG were increased in neurons under various preconditioning including
ischemic-like-reperfusion, hyperbaric oxygen, isoflurane and lipopolysaccharide in vitro. The growth of neurons under
ischemic-like-reperfusion pretreatment was significantly deceased in LRG expression silencing cells. In addition, the mRNA and protein
levels of LRG in brain tissues of mouse model of ischemia tolerance under various preconditioning were also up-regulated.Conclusion:Ischemia-reperfusion-like and various preconditioning promoted the expression of LRG in mice neurons. However, the precise molecular
mechanismof LRG in regulating cerebral ischemia-reperfusion injury remains unclear which deserved further investigation. |
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