| 杨亮 乔振奎 邴建勇 奚晓龙 朴庆华.基质金属蛋白酶和组织金属蛋白酶抑制剂在肾细胞癌组织中的表达[J].现代生物医学进展英文版,2014,14(13):2446-2449. |
| 基质金属蛋白酶和组织金属蛋白酶抑制剂在肾细胞癌组织中的表达 |
| Expression of Tissue levels of Matrix Metalloproteinases and TissueInhibitors of Metalloproteinases in Renal cell Carcinoma |
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| DOI： |
| 中文关键词: 基质金属蛋白酶 组织金属蛋白酶抑制剂 肾细胞癌 实时定量逆转录聚合酶反应 |
| 英文关键词: Matrix metalloproteinase Tissue inhibitor of metalloproteinase Renal cell carcinoma RT-PCR |
| 基金项目:黑龙江省教育厅科学技术研究项目(12511228) |
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| 中文摘要: |
| 目的：基质金属蛋白酶及组织金属蛋白酶抑制剂在肾细胞癌转移中占有重要的作用，研究肾细胞癌组织中MMP-2、
MMP-9、TIMP-1 和TIMP-2 的表达情况，为肾癌转移的治疗提供理论依据。方法：选取36 例肾细胞癌肾组织标本，从相同的肾细
胞癌组织及癌旁肾组织获得对照样本，均进行根治性肾切除手术切除。肿瘤分期按TNM分期标准。为了统计评估，肿瘤1 期和2
期为低级，3 期以上为高级。RT-PCR 检测肿瘤和正常组织中的MMP-2、MMP-9、TIMP-1 和TIMP-2 的表达。结果：不同样本
MMPs 和TIMPs 表达水平各不相同。肾细胞癌组织中MMP-2、MMP-9、TIMP-1、TIMP-2 在肾细胞癌中的表达明显高于正常肾组
织（P＜0.05）。在肾细胞癌的肿瘤分期方面，MMP-2 与MMP-9 和肿瘤的分期显著相关，随着肿瘤分期的增加，MMP-2 与MMP-9
的表达明显升高（P＜0.05），而TIMP-1 与TIMP-2 与肿瘤的分期无关。结论：肾细胞癌组织中TIMP-2、MMP-2，MMP-9，TIMP-1
的mRNA 表达显著高于正常肾组织，抑制MMPS 的表达将成为治疗肾细胞癌转移的新的方向。 |
| 英文摘要: |
| Objective:Matrix metalloproteinases and tissue inhibitors of metalloproteinases play an important role in renal cell
carcinoma. To examine the expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 in renal tissue samples of renal cell cancer, and To
provide a theoretical basis for the treatment of renal cell carcinoma metastasis.Methods: 36 cases of renal cell carcinoma specimens and
normal samples were fully obtained from the same renal cell carcinoma tissue and tumor adjacent renal tissues, those were performed
radical resection operation resection of kidney. Tumor staging according to TNMstaging. For statistical evaluation, tumor 1 and 2 stage
were determined lower grade, tumor 3 and senior stage were determined higher grade. Semi quantitative reverse transcription polymerase
chain reaction (RT-PCR) to detect the expression of tumor and normal tissues of MMP-2, MMP-9, TIMP-1 and TIMP-2.Results:MMPs
and TIMPs expression levels in different samples was different. Mean MMP-2, MMP-9, TIMP-1 and TIMP-2 mRNA expression in the
renal cell carcinomas was significantly higher than those in the normal renal tissue (P<0.05). In renal cell cancer staging, MMP-2 and
MMP-9 and tumor stage was significantly correlated with the increase of staging of tumor, MMP-2 and MMP-9 mRNA expression
increased significantly with the TNM stage of the tumor (P<0.05).Conclusion:Mean MMP-2, MMP-9, TIMP-1 and TIMP-2 mRNA
expression in the renal cell carcinomas was significantly higher than in the normal renal tissue, the inhibition of MMPS expression will
become the treatment of renal cell carcinoma metastatic to the new direction. |
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