| 田甜 温秋婷 杨薇薇 李春红 郑芳 蒋淑婉 李云 刘端阳1
曲雪梅 陈鹤.FAP-alpaha在胞外信号传递中与整合素的关系[J].现代生物医学进展英文版,2014,14(11):2058-2061. |
| FAP-alpaha在胞外信号传递中与整合素的关系 |
| The Relationship between FAP-alpha in Extracellular Signal Transduction andIntegri |
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| DOI： |
| 中文关键词: 成纤维细胞激活蛋白（FAP） 卵巢癌细胞系（HO-8910PM） 整合素α3β1 尿激酶型纤溶酶原激活剂受体(uPAR) |
| 英文关键词: Fibroblast activation protein (FAP) Ovarian cancer cell line (HO-8910PM) Integrin α3β1, urokinase-type plasminogen
activator receptor (uPAR) |
| 基金项目:黑龙江省自然科学基金（D201049） |
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| 中文摘要: |
| 目的: 研究成纤维激活蛋白（Fibroblast Activation Protein, FAP）在促进卵巢癌细胞发生侵袭、迁移、增殖过程中与整合素
α3β1、尿激酶型纤溶酶原激活剂受体( uPAR)的关系。方法：1）.免疫共沉淀共同检测整合素α3β1、uPAR在HO-8910PM上是否为
二聚体。2). transwell 侵袭实验、迁移实验检测抑制整合素α3β1、uPAR后，卵巢癌细胞系HO-8910PM的侵袭迁移能力；3）. 抑制
整合素α3β1、uPAR 后，再给予FAP琢对HO-8910PM侵袭、迁移和增殖的影响。结果：1).整合素琢3茁1、uPAR在HO-8910PM细胞
外同一个位置表达；2）.抑制整合素α3β1 能够明显抑制HO-8910PM的侵袭、迁移、增殖能力，并且可以抑制FAP 对肿瘤的作用。
3）. PAI-1 抑制uPAR后，HO-8910PM的侵袭、迁移、增殖无明显变化，同时对FAP 也无明显作用。结论：整合素α3β1 和uPAR在
HO-8910PM 是一个复合体，FAPα在细胞外是通过整合素α3β1 传递信号进入细胞内而不是通过uPAR，整合素α3β1 是通过
uPAR 与肿瘤细胞相连接 |
| 英文摘要: |
| Objective: To study the relationship of integrin α3β1, urokinase-type plasminogen activator receptor (uPAR) and
fibroblast activation protein (Fibroblast Activation Protein, FAP) in promoting invasion, migration, proliferation of ovarian cancer cells.Methods:1) Co-immunoprecipitation was used to detected to whether integrinα3β1 uPAR was a trimer of HO-8910PM; 2) Transwell
invasion assay, migration assay and MTT assay were to detect the impact on the invasion, migration and proliferation of HO-8910PM,
which imposed by inhibition of integrin α3β1, uPAR alone and with combined effects of FAP; 3）Inhibition of integrin α3β1, uPAR, and
then give FAPαon HO-8910PM invasion, migration and proliferation. Results: 1）Integrin α3β1, uPAR in HO-8910PM expression of
extracellular same position; 2) Inhibiting integrin α3β1 can inhibit HO-8910PM invasion, migration, proliferation, and can inhibit the
FAPα' function; 3) PAI-1 inhibition of uPAR, the ability of HO-8910PM invasion, migration, proliferation reduced and didn't have any
significant changes on the FAPα' function.Conclusion:Integrinα3β1 and uPAR in HO-8910PMis a complex, FAPαin the extracellular
pass through integrin α3β1 signal into the cell rather than through uPAR, integrins α3β1 by uPARconnected with tumor cells. |
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