| 宋颖韬1 周晓燕2 孙长生3 孟琰4 赵尔飏4△.错配修复蛋白hMSH2、hMLH1在涎腺粘液表皮样癌中的表达*[J].现代生物医学进展英文版,2014,14(10):1839-1843. |
| 错配修复蛋白hMSH2、hMLH1在涎腺粘液表皮样癌中的表达* |
| Expression of hMLH1, hMSH2 in Salivary Gland MucoepidermoidCarcinoma* |
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| DOI： |
| 中文关键词: 错配修复 hMSH2 hMLH1 涎腺 黏液表皮样癌 免疫组化染色 |
| 英文关键词: Mismatch repair hMSH2 hMLH1 Salivary gland Mucoepidermoid carcinoma Immunohistochemistry |
| 基金项目:黑龙江省自然科学基金项目（D201151） |
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| 中文摘要: |
| 摘要目的：探讨人类错配修复基因2 the human mutS homolog 2 (hMSH2)人类错配修复基因1human mutL homolog 1 (hMLH1)在
涎腺粘液表皮样癌（salivary gland mucoepidermoid carcinoma-SMEC）表达水平及临床病理意义。重点研究人类错配修复基因2 和
人类错配修复基因1与目标肿瘤发生的相关性。方法：采用HE染色方法筛选共计47 例典型病例，采用免疫组织化学染色分析
37 例SMEC、10 例正常组织涎腺中hMSH2、hMLH1 的表达水平，结合计算机辅助高清晰图像分析处理技术做出综合评价。结果：
hMLH1 的表达与SMEC分化呈负相关（P<0.05）；hMLH1 的低表达或表达缺失在低分化的SMEC 中较普遍，在中分化和高分化
中表达逐步增强；hMSH2 的表达与SMEC分化不相关（P>0.05）。结论：hMSH2、hMLH1异常表达与涎腺黏液表皮样的发生、演进
存在相关性，以hMLH1、hMSH2 为切入点为涎腺黏液表皮样癌治疗与预防提供参考依据。 |
| 英文摘要: |
| ABSTRACT Objective：The aim of this study was to describe the human mutS homolog2 (hMSH2) and human mutL homolog 1
(hMLH1) immunoexpression in different areas of salivary gland mucoepidermoid carcinoma in an attempt to verify if the histological
grade of malignancy interferes in this expression. The research emphasis is whether hMSH2, hMLH1 correlate with target carcinoma.
Methods:Tissues in 37 cases with mucoepidermoid carcinoma and 10 normal salivary gland were determined by HE staining and the expression
of hMLH1, hMSH2 were tested using computer-assised high-resolution image analysis and processing techniques. Results:The
hMHL1 immunoexpression was negatively correlated with histological grades of malignancy（P<0.05）; hMHL1 reduction or negative expression
was detected in poorly differentiated tumors, but the expression gradually enhanced in moderately differentiated and well differentiated
tumors. The hMSH2 immunoexpression was not correlated with histological grades of malignancy, whereas hMLH1 overexpression
was correlated and associated（P>0.05）with histological grades of malignancy.Conclusion: The anomalo expression of hMLH1,
hMSH2 get the relationship with the occurrence and development of slivary gland mucoepidermoid carcinoma. We can use hMLH1,
hMSH2 as a point to incisivus in slivary gland tumour, and they have graveness significance on the precaution and therapy to slivary
gland mucoepidermoid carcinoma. |
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