| 张卓超李霄马奔纪洪辰王璟阮柏于泳王德盛陶开山△ 窦科峰△.低剂量LPS 预处理的间充质干细胞对胰岛保护作用的研究*[J].现代生物医学进展英文版,2014,14(10):1808-1812. |
| 低剂量LPS 预处理的间充质干细胞对胰岛保护作用的研究* |
| Co-transplantation with LPS Preconditioned Mesenchymal Stem CellsPromote the Efficiency of Islets Grafts* |
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| DOI： |
| 中文关键词: 脂多糖 间充质干细胞 预处理 胰岛移植 |
| 英文关键词: Lipopolysaccharide Mesenchymal stemcells Precondition Islets transplantation |
| 基金项目:国家高技术研究发展计划“863”计划项目( 2012AA021005)；国家自然科学基金(81270549；81300361)； |
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| 中文摘要: |
| 摘要目的：探讨低剂量脂多糖(LPS)预处理的间充质干细胞(MSCs)对于胰岛移植物的保护作用及其机制。方法：给予MSCs不同
浓度的LPS 预处理，利用流式细胞仪检测不同处理组的间充质干细胞在低氧条件下的凋亡情况，筛选出最佳刺激浓度。通过
ELISA 检测低氧条件下LPS预处理组与未处理组的MSCs 促生长因子的分泌情况。利用Western blot 的方法检测不同处理组的
MSCs 在低氧条件下bax,bcl-2 的表达。体外低氧条件下共培养不同处理的MSCs和胰岛细胞，检测胰岛细胞内部CD31阳性细胞
的表达。以F344 大鼠为供者，以Balb/c 裸鼠为受者，制作胰岛联合MSCs移植模型,连续观察21 天检测胰岛功能的恢复情况。结
果：当以500ng/mL的LPS 刺激MSCs 能够减少MSCs 在低氧条件下的凋亡水平（P<0.05），此时为最适浓度。LPS预处理的MSCs
相较于未处理组能够在低氧条件下分泌更多的HGF，IGF-1，VEGF。LPS 预处理的MSCs 能够上调bcl-2 下调bax 的表达（P<0.
05）。LPS预处理的MSCs 能够明显保护低氧条件下胰岛细胞内部的CD31 阳性的内皮细胞的数量。胰岛细胞联合LPS 预处理
的MSCs能够明显提高胰岛功能的改善。结论：胰岛细胞联合LPS预处理的MSCs能够明显提高移植效率。 |
| 英文摘要: |
| ABSTRACT Objective：To investigate the effect of LPS preconditioned MSCs on the transplantation efficiency of islets grafts and
the possible mechanism. Merthods： Different concentration of LPS was used to precondition the MSCs. Apoptosis rates were measured by
the FACS, and the optimum concentration of LPS was determined by the lowest apoptosis rate under hypoxia. ELISA was used to detect
the secretion of growth factor by the MSCs. Western blot and PCR were used to detect the expression of the bax and bcl-2. In vitro,
MSCs was co-cultured with islets under hypoxic condition. CD31 expression in the intraislets was detected by the immunofluorescence.
xenogenic islets transplantation models were made using C57BL/6 as donor mice and Balb/c nude as recipient mice. Grafts function was
measured by the level of blood glucose. Results：500ng/ml was the optimum concentration to precondition MSCs. Pretreated with LPS
could reduce the apoptosis of MSCs under hypoxic condtion. Compared with that in the non-treatment group, the MSCs preconditioned
with LPS secret more HGF, IGF-1 and VEGF. In vitro results showed that MSCs preconditioned with LPS could preserve the CD31
positive cells in the intraislets under hypixa. Co-transplantation with LPS preconditioned MSCs could hasten the recovery of the islets
grafts.Conclusion：MSCs preconditioned with low dose of LPS could improve the efficiency of islets grafts. |
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