| 邱新毓 杨建栋 张毅 李燕 李成花 张兰慧 刘新平 郑瑾.NDRG2 通过调控CD24 影响肝癌细胞侵袭能力的研究[J].现代生物医学进展英文版,2014,14(9):1637-1640. |
| NDRG2 通过调控CD24 影响肝癌细胞侵袭能力的研究 |
| NDRG2 Expression Regulates CD24 and Invasion ability of Hepatocellularcarcinoma Cells |
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| DOI： |
| 中文关键词: NDRG2 CD24 肝癌细胞 侵袭 |
| 英文关键词: NDRG2 CD24 Hepatocellular carcinoma cell Adhesion |
| 基金项目:国家自然科学基金项目（81072973） |
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| 中文摘要: |
| 目的：阐明NDRG2（N-Myc downstream-regulated gene 2）在肝癌细胞中对CD24 的调控及其对乳腺癌细胞侵袭能力的影响。
方法：Western blot 检测低转移性的肝癌细胞Huh7、高转移性的肝癌细胞系MHCC97h 及正常人肝细胞系L-02 中NDRG2 和
CD24 的表达；通过腺病毒载体上调MHCC97h 细胞中NDRG2 的水平，或利用siRNA 下调Huh7 细胞中NDRG2 的表达，检测
CD24 的变化以及细胞侵袭能力的改变。结果：MHCC97h 细胞中NDRG2 基因和蛋白的表达水平低于Huh7 细胞,而CD24 的表达
水平高于Huh7 细胞；在MHCC97h 细胞中上调NDRG2 可以抑制CD24 的表达并抑制其侵袭能力，而在Huh7 细胞中下调
NDRG2 的表达可以提高CD24 的水平及细胞的侵袭能力。结论：NDRG2 可能通过影响CD24 参与调控肝癌细胞的侵袭能力。 |
| 英文摘要: |
| Objective:To explore the regulative role of N-Myc downstream-regulated gene2 (NDRG2) on CD24 expression and
the invasion ability of hepatocellular carcinoma cells.Methods:The mRNA and protein expressions of CD24 and NDRG2 in MHCC97H,
Huh7 and L-02 cells were analyzed. Changes in cell invasion were detected when NDRG2 was up- or down-regulated by adenovirus or
siRNA. The expression pattern of NDRG2 and CD24 in HCC cells and the relationship between NDRG2 and invision features were analyzed.Results:NDRG2 expression was negatively correlated with malignancy in HCC. CD24 protein decreased when the hepatocellular
cacinoma cell MHCC97H was infected by Ad-NDRG2. CD24 protein increased when the hepatocellular cacinoma cell Huh7 was transfected
by siRNA-NDRG2. The up-regulation of NDRG2 decreased CD24 expression and cell invasion. By contrast, the down-regulation
of NDRG2 enhanced CD24 expression and invasion.Conclusion:The results suggest that NDRG2 exerted anti-tumor activity by regulating
CD24, which mediates that cell-cell interaction, tumor proliferation and adhesion. |
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