| 刘广欣1 党立力1 黄启科1 周亮2 张福琴1 陶开山1△.大鼠门静脉分支残端置管模型构建及细胞移植途径的评价*[J].现代生物医学进展英文版,2014,14(8):1928-1930. |
| 大鼠门静脉分支残端置管模型构建及细胞移植途径的评价* |
| Establishing a Model of Acute Hepatic Failure with Catheterization of Portalvenous Branch and a Study of the Way of Cell Transplantation* |
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| DOI： |
| 中文关键词: 动物模型 门静脉置管 干细胞移植 胎肝干细胞 动物实验 |
| 英文关键词: Model of animal Catheterization of portal venous Stem cell transp1antation Fetal liver stem/progenitor cells Animal
experimentation |
| 基金项目:国家自然科学基金项目(43411C1471；43411E1912) |
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| 中文摘要: |
| 摘要目的：大鼠肝部分切除模型被广泛的应用于肝脏疾病的研究，随着干细胞治疗肝损伤及护肝药物研究的发展，对大鼠肝损伤
模型也提出了很多新的要求。本实验拟在大鼠肝部分切除术的基础上改进以建立大鼠肝断面门静脉分支残端的静脉置管模型，并
进行细胞移植实验，对比分析新模型的优劣。方法：60 只F344 大鼠分为三组。A、B 组行行85 %肝切除术；C组行85 %肝切除术+
肝断面门静脉分支残端置管术。术中B 组经门静脉注入4× 105个表达GFP（green fluorescence protein, GFP）的胎肝干细胞（fetal
liver stem/progenitor cells, FLSPCs）。C组经留置导管注射入同等量的FLSPCs，A组注射同等剂量的培养液。72小时取血清，测定
肝功能ALT、AST，统计死亡率；取肝脏组织切片观察其修复情况。统计学采用方差分析和LSD-t 检验。结果：B、C组F344 大鼠
72 小时肝功指标（ALT、AST）均明显优于A 组；B 组、C 组肝脏组织学的病理损伤的恢复分别较A 组快。B、C组间肝功指标无统
计学意义。结论：经门静脉分支残端置管途径移植FLSPCs 效果等同于经门静脉穿刺途径，且该模型具有可反复、可选时、减少创
伤等优点。 |
| 英文摘要: |
| ABSTRACT Objective:Rat partial hepatectomy model is widely used in the study of hepatic disease. As the rapid development of
research of liver protection drugs and stem cells therapy for liver injury, the model need to be improved. A rat model of acute hepatic
failure with catheterization of portal venous branch was established . After a study of the way of cell transplantation was made in our trail,
the new model was evaluated. Methods:Sixty F344 rats were randomly divided into 3 groups (n=20 in each group). The partial
hepatectomy with 85% sizes were performed on the rats of the A and B groups. The same operations and catheterizations of portal
venous branch were carried out on the rats of the C group. After the green fluorescent protein (GFP) gene was transferred into the
FLSPCs, Every rats of the B group were transplanted into 4× l05 cells through the portal veins during the operations. Every rats of the C
group were transplanted into the same cells through the ducts. Control rats in the A group received medium of equal volumes. Mortality
rates and hepatic functions such as ALT AST were measured after 72 hours. Meanwhile, hepatic pathology was studied. Data were
analyzed using variance analysis and LSD-t. Results：Compared to control group the hepatic and coagulative functions gradually
recovered in stem ce1ls transplant groups(B and C). The hepatic pathology significantly improved in stem ce1ls transplantation groups(B
and C). The effects of treatments on the rats ofB group are equal with those of C group. Conclusion：Stem cells transplantations through
ducts of portal venous branch improved the hepatic functions and pathology in acute hepatic injury model of F344 rats as much as those
through portal vein directly. The model has advantages in repeated operation, fewer traumas and less complication and can be treated
whenever necessary. |
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