| 胡彬1，2，3 王赫2，3 晏贤春2，3 蒋芳2，3 王清明2，3 杨俊涛2，3△.高脂培养抑制LPS诱导的HSC-T6 细胞活化*[J].现代生物医学进展英文版,2014,14(8):1420-1422. |
| 高脂培养抑制LPS诱导的HSC-T6 细胞活化* |
| The Fat Culture Inhabited LPS-Induced Activation of HSCs-T6 Cells* |
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| DOI： |
| 中文关键词: 游离脂肪酸 肝星形细胞 LPS 肝纤维化 |
| 英文关键词: Free fatty acids HSC-T6 LPS Liver fibrosis |
| 基金项目:国家自然科学基金项目(31000405)；国家高技术研究发展计划（2012AA020201）； |
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| 中文摘要: |
| 摘要目的：鉴定高脂培养对肝星形细胞活化的影响。方法：培养HSC-T6 细胞系，加入含有游离脂肪酸的高脂培养基处理，利用
LPS 处理活化，通过检测α-SMA 的表达分析星形细胞的活化程度，通过Q-PCR分析HSCs 细胞胶原的表达，通过Q-PCR实验分
析LPS相关通路靶基因表达情况情况。结果：高脂培养能够抑制LPS 诱导的HSC-T6 细胞增殖，降低HSC-T6 细胞α-SMA 和胶
原I和TIMP-1 表达的水平，Q-PCR 的分析表明，高脂培养能够抑制HSCs活化后的NF-κB 通路下游靶基因MCP-1 和IL-6 的表
达。结论：在体外培养实验中，高脂培养能够抑制LPS诱导的HSC-T6 细胞活化。 |
| 英文摘要: |
| ABSTRACT Objective：To investigate the effect of high-fat medium on the activition of hepatic stellate cells. Methods：HSC-T6
cell line was cultured, and treated with high-fat medium which containing free fatty acid, activated with LPS. The degree of activation
was analyzed by detecting α-SMA expression. The expression of collagen-I and TIMP-1 was analyzed by Q-PCR, and the activation of
transcription factor which related to LPS pathway was analyzed by Q-PCR assay. Results：High-fat medium was able to inhibit the
activation of HSC-T6 cells which induced by LPS, reduced the expression of collagen level in the HSC-T6 cells. Q-PCR analysis showed
that the high-fat medium inhibited the expression of MCP-1 and IL-6 which were the target genes of NF-κB in activated HSCs.
Conclusion：In vitro culture experiments, high-fat mediumcan inhibit the activation of HSC-T6 cells which induced by LPS. |
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