| 王莉汪廷乐欧学平宋萌△ 潘劲松△.TGF-β1 诱导人牙周膜细胞细胞骨架重排的机制*[J].现代生物医学进展英文版,2014,14(8):1415-1419. |
| TGF-β1 诱导人牙周膜细胞细胞骨架重排的机制* |
| The Mechanismof TGF-β1-Induced Cytoskeletal Rearrangement ofHuman Periodontal Ligament Cells* |
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| DOI： |
| 中文关键词: 转化生长因子-β1 人牙周膜细胞 细胞骨架重排 Rho 信号通路 |
| 英文关键词: TGF-β1 Human Periodontal Ligament Cells Cytoskeletal Rearrangement Rho signaling pathway |
| 基金项目:国家自然科学基金项目（11172177）；上海交通大学医工理交叉基金项目（YG2010MS07） |
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| 中文摘要: |
| 摘要目的：牙周病是由多种因素引起的，特别是人牙周膜细胞的缺失。转化生长因子-β1（TGF-β1）是一种多功能细胞因子，在治
疗牙周病中发挥重要的作用，但很少有人清楚地研究TGF-β1 对人牙周膜细胞的影响。因此，本研究的目的是探讨TGF-β1诱导
人牙周膜细胞细胞骨架重排的信号通路。方法：人牙周膜细胞取自健康的前磨牙，并向同步化处理的细胞中加入10 ng / ml 的
TGF-β1，并通过相差显微镜观察它们的形态学变化。通过免疫组化和共聚焦显微镜观察F-肌动蛋白重排。用Western blot 分析蛋
白表达情况。结果：我们发现TGF-β1 诱导人牙周膜细胞细胞骨架重排，激活ROCK 蛋白的表达，并增加p-lIMK 和p-cofilin 的
蛋白表达。ROCK 抑制剂Y-27632 使ROCK，p-lIMK 和p-cofilin 的蛋白表达下降。结论：TGF-β1可以诱导人牙周膜细胞细胞骨架
重排，并且是通过上凋ROCK，p-lIMK 和p-cofilin 的活性完成的。本研究可以增强对TGF-β1 在治疗牙周疾病方面的作用机制的
了解。 |
| 英文摘要: |
| ABSTRACT Objective：Periodontal disease caused by a variety of factors, particularly the loss of hPDLCs. Transforming growth
factor-β1 (TGF-β1) is a multifunctional cytokine known to play an important role in periodontal disease, but little is known about the effects
of TGF-β1 on human PDL cells. The aim of our study is to elucidate the signaling pathway that mediates these effects.
Methods：The human PDL cells were obtained from healthy premolars. Serum-starved hPDLCs were incubated with 10 ng/ml TGF-β1, their morphological
changes were examined by phase-contrast microscopy. F-actin reorganization was observed by immunochemistry and confocal
microscopy. Protein expression was analyzed by Western Blot analysis.Results： TGF-β1 treatment induced cytoskeletal reorganization,
activated ROCK protein expression, and increased the phosphorylation of LIM kinase and cofilin. Additionally, protein expression
of ROCK and the phosphorylation of LIM kinase and cofilin were decreased by the ROCK inhibitor Y-27632.Conclusion： TGF-β1 can
induce hPDLCs to undergo cytoskeletal rearrangement, and it is through upregulating ROCK, p-LIM kinase and p-cofilin activity. This
study may help our better understanding of the mechanismof TGF-茁1 in curing periodontal disease. |
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