| 王莹 王鸿 蔡东联 张玉珍 黄莺.高脂饮食诱导肥胖与肥胖抵抗型非酒精性脂肪肝
大鼠模型的建立[J].现代生物医学进展英文版,2014,14(7):1221-1224. |
| 高脂饮食诱导肥胖与肥胖抵抗型非酒精性脂肪肝
大鼠模型的建立 |
| Induction Method of Non-Alcoholic Fatty Liver Modelin Obesity and Obesity-Resistant Rats |
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| DOI： |
| 中文关键词: 肥胖抵抗 非酒精性脂肪肝 大鼠 动物模型 |
| 英文关键词: Obesity-resistant Non-alcoholic fatty liver Rats Animal model |
| 基金项目:上海市科委基金项目（024119048） |
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| 中文摘要: |
| 目的：利用高脂饲料复制肥胖与肥胖抵抗型非酒精性脂肪肝SD 大鼠模型。方法：体质量100± 10g的雄性SD大鼠140 只，
按照体重随机抽取120 只用于模型建立，喂食高脂、高能饲料。连续8 周后，将体质量大于正常对照组平均体质量+1.96 倍标准差
的模型大鼠作为肥胖型非酒精性脂肪肝组（NO组），体质量小于正常对照组平均体质量+1.0 倍标准差的作为肥胖抵抗型非酒精
性脂肪肝组（NOR 组）。8 周内动态观察大鼠的一般情况、体质量变化，8 周末每组随机取8 只处死，比较血清谷丙转氨酶（ALT）、
谷草转氨酶（AST）、胆固醇(TC)、甘油三脂(TG)水平变化及肝指数、脂体比，观察肝脏形态学改变。剩余20 只作为正常对照组，喂
食普通饲料。结果：NO与NOR组大鼠体重增长差距逐渐增大，至8w末，NO 组体重显著高于NOR 组及正常对照组(P <0.01)，脂
肪重量和脂体比均显著升高，NO组脂肪重量显著高于NOR组（P <0.05，0.01），但脂体比间未见显著差异；NO 与NOR组TG、
ALT 显著升高（P <0.05），其中NO 组大鼠血清TG、TC显著高于NOR组（P <0.05）；两组肝重量和肝指数均显著升高，NO 组肝重
量显著高于NOR组（P <0.05，0.01），但肝指数间未见显著差异，两组肝细胞内均弥散大量脂肪空泡。结论：利用高脂饲料成功建
立肥胖与肥胖抵抗型非酒精性脂肪肝SD 大鼠模型，与人类发病特征相似，为肥胖与非酒精性脂肪的研究提供更有针对性的动物
模型。 |
| 英文摘要: |
| Objective:To establish obesity and obesity-resistant rats model of non-alcoholic fatty liver.Methods: 140 male Sprague
Dawley rats were randomly divide into group control (20) and model (120), maintained a standard diet and high-energy-fat diet for 8
weeks, respectively. Then the rats of high-energy-fat group were divided into 2 groups s: NO and NOR groups. The rats of body mass
higher than nomal +1.96 times standard deviation were divided into NO group and the rats of body mass higher than nomal +1.0 times
standard deviation were divided into NOR group. The general conditions and weight changes were dynamically observed for 8 weeks,
and then killed 8 rats of different group. The pathological changes of liver tissues were observed by HE staining. The following indexes
were compared among the three groups, including serum levels of triglyceride (TG), total cholesterol (TC), alanine aminotransferase
(ALT), aspartate aminotransferase (AST) and liver index, body fat ratio.Results:Conclusion:Starting from the fifth week，the weights of rats were
significantly decreased in group control and NOR as compared with those in group NO (P <0.01). The serum levels of ALT, TG, liver
weight and index, body fat ratio in group NO and NOR were markedly higher than control (P <0.05). The TC, TG levels and weight of
liver in group NO were increased significantly compared with group NOR (P <0.05). And there was no significantly difference between
group NO and NOR in liver index and body fat ratio. Light microscopy showed a great number of fat vacuoles in liver cell of group NO
and NOR. |
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