| 于囡 王海桃 杜鹃 李荣 刘赛.扇贝糖胺聚糖对感染HSV-Ⅰ小鼠免疫功能的影响研究[J].现代生物医学进展英文版,2014,14(4):668-670. |
| 扇贝糖胺聚糖对感染HSV-Ⅰ小鼠免疫功能的影响研究 |
| The Effect of Scallop Skirt Glycosaminoglycan on Immune Functionin Mice Infected with HSV-Ⅰ |
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| DOI： |
| 中文关键词: 扇贝裙边 糖胺聚糖 单纯疱疹病毒Ⅰ型 免疫调节 |
| 英文关键词: Scallop skirt Glycosaminoglycan HSV-I Immune regulation |
| 基金项目:山东省中医药发展科技项目（2005-16） |
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| 中文摘要: |
| 目的：研究扇贝裙边糖胺聚糖(glycosaminoglycan fromScallop Skirt, SS-GAG)对感染单纯疱疹病毒Ⅰ型(herpes simplex virus
type, HSV-Ⅰ)小鼠免疫功能的影响。方法：通过在无菌条件下给予小鼠注射扇贝糖胺聚糖SS-GAG,连续11 天，并在给药第三天
给小鼠腹腔注射HSV-Ⅰ病毒悬液建立小鼠感染模型，用MTT 等方法观察SS-GAG对HSV-Ⅰ感染小鼠腹腔巨噬细胞吞噬活性
的影响、对脾脏指数、胸腺指数的影响以及对脾淋巴细胞转化能力等免疫指标的影响。结果：与病毒对照组相比，扇贝糖胺聚糖
SS-GAG低剂量组、中剂量组、高剂量组均能显著增强HSV-Ⅰ感染小鼠的腹腔巨噬细胞的吞噬活性和HSV-Ⅰ感染小鼠的脾脏指
数和胸腺指数(P<0.01)，并且能促进其脾淋巴细胞转化增殖能力(P<0.01)。结论：扇贝糖胺聚糖在体内有一定的抗Ⅰ型单纯疱疹病
毒作用。其抗病毒作用可能与增强机体免疫功能有关。 |
| 英文摘要: |
| Objective:To investigate the effect of scallop skirt glycosaminoglycan on immune function in mice infected by HSV-I.Methods:Every mouse was treated by SS-GAG with different doses for 11 days. After 72 hours, the mice were injected by HSV-I. The
effects of SS-GAG on the phagocytic activity of peritoneal macrophage and the proliferation of splenic lymphocyte were studied by MTT
colorimetry. The index of spleen and thymus were used to study the influences of SS-GAG on function of the immune organs.Results:Compared with virus control, SS-GAG (10 mg/kg, 20 mg/kg and 40 mg/kg) could significantly enhance phagocytic activity of peritoneal
macrophage in mice infected by HSV-I, and promote proliferation of splenic lymphocyte induced by concanavalina and lipopolysaccharide
(P<0.01). Meanwhile, the index of thymus of mice treated by SS-GAG was improved significantly (P<0.01).Conclusion:The
glycosaminoglycan from scallop skirt (SS-GAG) has the depressant effect on HSV-I in vivo, and the mechanism of anti-virus may
attribute to improving the immune function of organs. |
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