| 彭程飞 王艳春 汪洁 邓捷 田孝祥 闫承慧 韩雅玲.人参皂苷Rg1 通过缺氧诱导因子1α促进血管内皮
生长因子旁分泌[J].现代生物医学进展英文版,2014,14(4):617-620. |
| 人参皂苷Rg1 通过缺氧诱导因子1α促进血管内皮
生长因子旁分泌 |
| Ginsenoside-Rg1 Promote VEGF Production ViaHypoxia-inducible Factor-1α |
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| DOI： |
| 中文关键词: 骨髓间充质干细胞 人参皂苷Rg1 缺氧诱导因子1α 血管内皮生长因子 |
| 英文关键词: MSCs Ginsenoside-Rg1 HIF-1α VEGF |
| 基金项目:国家自然科学基金项目(30770793，30971218，81070097)；国家青年科学基金项目（30800465） |
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| 中文摘要: |
| 目的：研究人参中提取的小分子活性物质皂苷Rg1 能否促进大鼠骨髓间充质干细胞（marrow-derived mesenchymal stem
cells, MSCs）分泌血管内皮生长因子，并进一步探究其作用机制与缺氧诱导因子1α（Hypoxia-inducible factor，HIF-1）的相关性。方
法：用酶联免疫吸附剂测定法（enzyme linked immunosorbent assay, ELISA）观察细胞培养上清中的血管内皮生长因子（vascular endothelial
growth factor，VEGF）以及蛋白印迹法（western blot）观察细胞中缺氧诱导因子相关蛋白的变化。结果：①通过对照组与人
参皂苷Rg1处理组比较发现，1 小时前血管内皮生长因子的分泌在两组之间没有明显差异，而在1 小时后人参皂苷Rg1 处理组
相比较于对照组的血管内皮生长因子分泌显著性增加；②进一步实验发现人参皂苷Rg1 处理的骨髓间充质干细胞中的HIF-1α
在1 小时后明显增加，而HIF-1β没有随时间有明显变化。结论：人参皂苷Rg1 具有促进骨髓间充质干细胞分泌VEGF的功能，并
且发现HIF-1α在这一过程中发挥关键作用，为将来人参皂苷Rg1 在临床中的广泛应用提供了重要的理论依据。 |
| 英文摘要: |
| Objective:To investigate the promotive effects of Ginsenoside-Rg1 on the marrow-derived mesenchymal stem cells
producing VEGF via HIF-1α.Methods:Mononuclear cells derived fromrat bone marrow were isolated by density-gradient centrifugation
and were cultured on fibronectin-coated plates, supplied with bovine pituitary extract. After Ginsenoside-Rg1acting on the marrow-derived
mesenchymal stem cells, we compared the production of VEGF by ELISA, the protein expression of HIF-1αin different groups by
western blot.Results:① the production of VEGF had significantly increased with Ginsenoside-Rg1. ② Rg1 induced a robust
accumulation of HIF-1αprotein in MSCs under normoxic conditions, which was clearly detectable after 1 h. In contrast, Rg1 did not
change levels of HIF-1βprotein.Conclusion:HIF-1αhad crucially effects on the marrow-derived mesenchymal stem cells for VEGF
production and it provides important theoretical basis for future ginsenoside Rg1 widely used in clinical. |
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