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目的：不可逆电穿孔是治疗肿瘤的新兴技术，本文探讨高压电场引起的不可逆电穿孔诱发A549 肺癌细胞凋亡的特点。方法：选择处于生长周期的A549细胞，共分为A-G7 个组进行研究，其中A组为不施加电场的空白对照组，B-G 组为实验组，B 组施加500 V/cm 强度高压电场，G 组施加1750 V/cm的高压电场，BG 组之间各组的高压电场强度间隔为250 V/cm。采取细胞抑制实验、不可逆电穿孔示踪实验、细胞凋亡实验，检验A549 细胞细胞凋亡与电场强度的关系。结果：①各实验组与对照组、各实验组之间的细胞抑制率，均存在显著性差异(P<0.05)；② 电场强度≥ 1000V/cm时，细胞不可逆电穿孔率明显增加，有统计学意义(P<0.05)；电场强度≥ 1500 V/cm 时，细胞不可逆电穿孔率增加不明显，无统计学意义(P＞0.05)；③ 电场强度≥ 1250 V/cm时，细胞早期凋亡率明显增加，有统计学意义(P<0.05)。结论：高压电场不可逆电穿孔诱发A549 肺癌细胞发生早期凋亡的强度为1250 V/cm，发生晚期凋亡的强度为1500 V/cm，且凋亡率随着电场强度的增加持续升高。这对于高压电场不可逆电穿孔效应引起的肿瘤细胞凋亡机制的研究具有重要意义。

英文摘要:

Objective:Irreversible Electroporation is the treatment of tumors of emerging technologies. Evaluatesthe characterof the apoptosis of A549 lung cancer cells induced by irreversible electroporation of high voltage electric field had been studied in this article.Methods:Selecting A549 lung carcinoma cells being in growth cycle. There were seven groups named A to G, and the control group is A without electric field. Exerting high voltage electric field of 500 V/cm to B and 1750 V/cm to G. The distance of high voltage elecric field is 250 V/cm. Cell inhibition assay, tracing the irreversible electroporation and apoptosis assays were taken to evaluate the relationhip between the apoptosis of A549 lung carcinoma cells with high voltage electric field.Results:① Significant differences of cell inhibition rate were found between each subgroup (P<0.05). ② Significant differences of irreversible electroporation rate were found when the electric field strength increased upon 1000 V/cm(P<0.05). No significant differences of irreversible electroporation rate were found when the electric field strength increased upon 1500 V/cm (P>0.05). ③ Apoptosis in prophase increased significantly when the electric field strength was greater than 1250 V/cm (P<0.05).Conclusion:Apoptosis in prophase appeared at the electric field strength of 1250 V/cm with irreversible electroporation. Apoptosis in advanced stage appeared at the electric field strength of 1500 V/cm with irreversible electroporation, and the apoptosis rate continuing to increase along with the electric field strength increasing. It was important that high voltage field induced the apoptosis of A549 lung carcinoma cells.

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