| 张捍军张睿李华哲勾旭升刘立冰赵承斌△.补骨脂抑制人工关节假体周围骨溶解的研究*[J].现代生物医学进展英文版,2014,14(2):247-250. |
| 补骨脂抑制人工关节假体周围骨溶解的研究* |
| Study on Fructus Psoraleae to Inhibit Periprosthetic Osteolysis* |
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| DOI： |
| 中文关键词: RANKL-RANK-OPG 系统 补骨脂 骨溶解 钛颗粒 人工关节置换术 |
| 英文关键词: RANKL-RANK-OPG system Fructus psoraleae Osteolysis Titaniumparticle Artificial joint replacement |
| 基金项目:黑龙江中医药科研项目（ZHY12-Z177） |
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| 中文摘要: |
| 摘要目的：探讨中药补骨脂对人工关节置换术后假体周围骨溶解，假体无菌性松动的核因子资B受体活化因子配体- 核因子资B
受体活化因子受体- 骨保护素(RANKL-RANK-OPG)信号通路的作用。方法：取24 只200± 5 g雌性SD大鼠，随机分为A、B、C
组，均注射钛颗粒溶液建立骨溶解模型，术后A 组采用中药补骨脂水溶液腹腔注射，B 组采用外源性OPG 腹腔注射作为阳性对
照，C 组采用生理盐水腹腔注射作为阴性对照。给药4 周后,通过免疫组化及反转录聚合酶链反应(RT-PCR)检测各组动物体内
RANKL/RANK的表达水平变化。结果：用药4 周后，3 组12 只大鼠背部气囊均为厚壁囊腔，骨溶解模型成功建立，免疫组化及
RT-PCR 检测RANKL/RANK 均呈阳性表达。免疫组化RANKL平均光密度(AOD)值A组(0.168± 0.017)、B 组(0.147± 0.009)明显
低于C 组(0.314± 0.011)，差异有统计学意义(P＜0.05)；A 组、B组之间比较无明显差异。RANK AOD值测定A组(0.172± 0.015)、
B 组(0.193± 0.045)明显低于C 组(0.342± 0.007)，差异有统计学意义(P＜0.05)；A 组、B 组之间比较无明显差异。RT-PCR 检测
RANKL相对量A 组(0.575± 0.143)、B 组(0.543± 0.174)明显低于C 组(0.951± 0.362)，差异有统计学意义(P＜0.05)；A 组、B 组之
间比较无明显差异。RANK 相对量A 组(0.433± 0.025)、B组(0.611± 0.209)明显低于C 组(0.871± 0.211)，差异有统计学意义(P＜
0.05)；A 组、B 组之间比较无明显差异。结论：中药补骨脂对人工关节置换术后假体周围骨溶解，假体无菌性松动具有抑制作用。 |
| 英文摘要: |
| ABSTRACT Objective:To investigate the effect of fructus psoraleae on RANKL-RANK-OPG signaling pathway of periprosthetic
osteolysis and aseptic loosening. Methods:Twenty-four 200± 5 g female SD rats were divided into three groups randomly, i.e. group A,
B and C. Each rat was injected titanium particle solution to establish the model of periprosthetic osteolysis. Rats in group A were used
fructus psoraleae solution for intraperitoneal injection from postoperation. Rats in group B were was injected ectogenic OPG as positive
control, and Rats in group C were injected normal saline as negative control. At the 4th week, the expression levels of RANKL and
RANK were detected by immunohistochemical and RT-PCR. Results:At the 4th week, dorsal gasbags of the 12 rats were thick-walled
ones, which mean the model of periprosthetic osteolysis established successfully. RANKL and RANK expressed positive in all the three
groups. RANKL average optical density (AOD) of Group A (0.168± 0.017) and Group B (0.147± 0.009) was significantly lower than
that in GroupC (0.314± 0.011) (P＜0.05). There was no evident difference between Group A and GroupB (P>0.05). RANK AOD of
Group A (0.172± 0.015) and GroupB (0.193± 0.045) was significantly lower than that in Group C (0.342± 0.007) (P＜0.05). There was
no difference between Group A and Group B (P>0.05). By the RT-PCR, RANKL relative quantity of Group A (0.575± 0.143) and Group
B (0.543± 0.174) was significantly lower than that in the Group C (0.951± 0.362) (P＜0.05). There was no difference between Group A
and GroupB (P>0.05). RANK relative quantity of GroupA (0.433± 0.025) and GroupB (0.611± 0.209) was significantly lower than
that in the GroupC (0.871± 0.211) (P＜0.05).Conclusion: There was no difference between Group A and GroupB (P>0.05). Fructus
psoraleae has inhibiting effect on periprosthetic osteolysis and aseptic loosening. |
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