| 栾丽霞李怡赵宏喜姜峰丁迎春刘成娟尹国武△.TGF-β1 和IGF-1 mRNA在早期胚胎停育绒毛组织中的表达及意义*[J].现代生物医学进展英文版,2014,14(2):240-243. |
| TGF-β1 和IGF-1 mRNA在早期胚胎停育绒毛组织中的表达及意义* |
| Study on Expression of TGF-β1 and IGF-1mRNA in the Chorionic Villi ofEarly Embryo Growth Arrest* |
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| DOI： |
| 中文关键词: 胚胎停育 绒毛组织 TGF-β1 IGF-1 RT-PCR |
| 英文关键词: Early embryo growth arrest Chorionic villi tissues TGF-β1 IGF-1 RT-PCR |
| 基金项目:国家自然科学基金项目（30973208；31000660） |
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| 中文摘要: |
| 摘要目的：通过检测胚胎停育患者绒毛组织中TGF-β1 和IGF-1 mRNA 的表达，探讨其在胚胎停育发病机制中的作用。方法：用
半定量逆转录聚合酶链反应（RT-PCR）技术检测正常人流（20 例），胚胎停育(25 例)绒毛组织中TGF-β1 和IGF-1 基因在转录水平
的表达。结果：（1）与对照组相比，实验组绒毛组织中TGF-β1mRNA表达量降低（P ＜0.05），（2）胚胎停育患者绒毛中IGF-1mRNA
表达量升高（P ＜0.05）。（3）绒毛组织中TGF-β1 与IGF-1 的表达呈负相关（r=-0.793，P ＜0.05）。结论：TGF-β1、IGF-1 的表达在基
因转录水平发生改变，TGF-β1 表达的降低可能上调了IGF-1 的表达，提示两者可能共同参与了胚胎停育的发生。 |
| 英文摘要: |
| ABSTRACT Objective:Onto investigate the expression of TGF-β1 and IGF-1 mRNA in the chorionic villi of early embryo growth
arrest in order to evaluate the role of early embryo growth arrest.Methods: Reverse transcription polymerase chain reaction (RT-RCR)
was used in the normal groups (20 cases ) and the early embryo growth arrest group (25 cases ) to quantitatively determining TGF-β1
mRNA and IGF-1 mRNA in the chorionic villi tissues. Their quantities were noted by the density of TGF-β1/β-actin or IGF-1/β-actin.
Results：(1) Compared with that in the normal group, the mRNA expression level of TGF-β1 of early embryo growth arrest was lower ( P
＜0.05), (2) The levels of IGF-1 was significantly higher (P ＜0.05). (3) There was a negative correlation between the TGF-β 1 and
IGF-1 in chorionic villi tissues( r=-0.793, P＜0.05). Conclusion：The abnormal expression of the TGF-β1 and IGF-1 was already changed
at the level of transcription. TGF-β1 may increase the expression of IGF-1 in some extent, which indicated that they might be involved in
the pathogesis of early embryo growth arrest. |
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