| 王霞周江睿蒋春雷△.神经肽Y 对炎症及低氧环境下不同癌细胞活力的影响[J].现代生物医学进展英文版,2012,12(27):5241-5243. |
| 神经肽Y 对炎症及低氧环境下不同癌细胞活力的影响 |
| The Effect of Neuropeptide Y on Inflammation and the Viability of theDifferent Carcinoma Cells under Hypoxia |
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| DOI： |
| 中文关键词: 神经肽Y 一氧化氮 炎症 肿瘤 低氧 |
| 英文关键词: Neuropeptide Nitric oxide Inflammation Carcinoma cells Hypoxia |
| 基金项目: |
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| 中文摘要: |
| 目的：研究神经肽Y 对炎症反应及其对在低氧培养条件下的不同癌细胞活力的影响，探讨应激影响癌症发展的机制。方法：
不同浓度神经肽Y（0，10-12M，10-10M，10-8M）与100 ng/ml LPS 共孵育巨噬细胞24h 后检测NO 的浓度及iNOS 表达的变化；不同
浓度神经肽Y（0，10-9M，10-8M）刺激低氧培养条件下的肝癌细胞株HepG2 与乳腺癌细胞株MCF-7 36h，采用cck-8 检测细胞活
力变化；不同浓度神经肽Y（0，10-9M，10-8M）与LPS 共孵育巨噬细胞24 h 后取上清液离心，采用上清液培养两种癌细胞，并置于
低氧条件下36 h, cck-8 检测细胞活力。结果：实验结果显示，神经肽Y 可以抑制巨噬细胞NO 的释放（P<0.05），并降低iNOS 的表
达(P<0.05)；单独的神经肽Y 对低氧培养条件下两种癌细胞的活力没有明显影响(P>0.05)；但在低氧培养条件中，相比于LPS 组
的条件培养基，LPS 加神经肽Y 组的条件培养基可明显增强MCF-7 的活力（P<0.05,P<0.01），而HepG 2 的活力则没有统计学差
异。结论：神经肽Y 可能通过抑制炎症反应，从而增强乳腺癌细胞MCF-7 在低氧环境下的活力。 |
| 英文摘要: |
| Objective: To investigate the effect of neuropeptide Y on inflammation and the viability of different carcinoma cells
under hypoxia condition and to investigate the mechanism of stress on carcinoma cells. Methods: Macrophage cells were incubated in
culture with different concentrations (0, 10-12M，10-10M，10-8M) NPY combined with 100 ng/ml LPS for 24 h and then detected the NO
release and iNOS expressions; HepG 2 and MCF-7 cells were incubated in culture with different concentrations (0, 10-9M，10-8M) NPY
under hypoxia condition (37 ℃, 5 % CO2，1% O2) for 36 h . The viability of carcinoma cells were in different groups detected by cck-8.
Macrophage cells were incubated in culture with different concentrateons (0, 10-9M，10-8M) NPY combined with 100ng/ml LPS for 24 h
and then the media were centrifuged. HepG 2 and MCF-7 cells were cultured with the conditioned media under hypoxia condition for 36
h. cck-8 was used for detecting the viability of carcinoma cells. Results: Neuropeptide Y markedly reduced the release of NO (P<0.05）
and inhibited the expression of iNOS (P<0.05). NPY had no obvious effects on the viability of HepG 2 and MCF-7 cells under hypoxia.
But the conditioned media which LPS and NPY treated macrophage markedly increased the viability of MCF-7 (P<0.01, P<0.05), and
had no effects on HepG 2. Conclusion: NPY inhibits macrophage cells releasing NO and inhibits the expression of iNOS and then
increases the viability of MCF-7 under hypoxia. |
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