| 曾婷马丽谢逸欣郝丽金星.大肠杆菌变种的两种分子生物学检测方法分析[J].现代生物医学进展英文版,2012,12(27):5238-5240. |
| 大肠杆菌变种的两种分子生物学检测方法分析 |
| Analysis of Escherichia Coli Variantsof the Molecular Biology Detection Methods |
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| DOI： |
| 中文关键词: 肠出血性大肠杆菌O157-H7 自动免疫磁珠收集系统 自动酶标免疫测试系统 |
| 英文关键词: Enterohemorrhagic Escherichia coli O157-H7 AIMS VIDAS |
| 基金项目: |
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| 中文摘要: |
| 目的：对大肠杆菌的一种重要的变种-- 肠出血性大肠杆菌O157-H7 的几种检测方法进行比较研究。方法：以自动免疫磁珠
收集系统（AIMS）、自动酶标免疫测试系统（VIDAS）与传统常规的分离方法进行对比分析。结果：运用自动免疫磁珠收集系统
（AIMS）方法对80 份可能含有肠出血性大肠杆菌O157-H7 的实样进行检测，检出份数为6 份，检出率为7.5%，而且在一周之内
可以全部对上述检出实样进行鉴定。AIMS 法能够检出浓度在10cfu/mL 模拟实样之中的肠出血性大肠杆菌O157-H7，然后将此
法与CHROMagar 0157 琼脂板相结合，其效果则更为明显。而自动酶标免疫测试系统（VIDAS）与传统与的分离方法则检测的效
果不佳，检出率为0。自动免疫磁珠收集系统（AIMS）检测方法与自动酶标免疫测试系统（VIDAS）、传统与的分离方法在检出率方
面存在显著的统计学差异，P＜0.01。结论：运用自动免疫磁珠收集系统（AIMS）结合CHROMagar 0157 琼脂板对出血性大肠杆菌
O157-H7 进行检测，检出率较高、灵敏度较高且快速便捷，可以用于O157-H7 外环境检测与食品污染源的实际调查之中，应该对
其加以广泛地推广并应用。 |
| 英文摘要: |
| Objective: Escherichia coli is a kind of important variants -- enterohemorrhagic Escherichia coli O157-H7 several
detection methods are comparative study. Methods: The automated immunomagnetic beads collection system ( AIMS ), automatic
enzyme immunoassay test system ( VIDAS ) and traditional and separation method for comparative analysis. Results: The use of
automated immunomagnetic beads collection system ( AIMS ) on80may contain enterohemorrhagic Escherichia coli O157-H7samples
for detection, detection of copy number is 6, the detection rate was 7.5%, and within a week to all of the above can be detected samples
for identification. AIMS method can detect concentration at 10cfu / mL simulation samples of enterohemorrhagic Escherichia coli
O157-H7, then this method and CHROMagar 0157agar plate combination, the effect is more obvious. Automatic enzyme immunoassay
test system ( VIDAS ) and traditional and separation method detection effect is poor, the detection rate is 0. Conclusion: The use of
automated immunomagnetic beads collection system ( AIMS ) combined with CHROMagar0157agar plates for hemorrhagic Escherichia
coli O157-H7were detected, the detection rate is higher, higher sensitivity and fast and convenient, can be used in O157-H7environment
detection and food contamination sources of practical investigation, should be widely promoted and applied. |
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