| 刘彬王凤军苏丹颖张惊宇陈修芬于美婷初婷婷刘欧侯丹慧张双彦.辛伐他汀对IFN-γ 诱导的人外周血单个核细胞OX40L 表达的影响[J].现代生物医学进展英文版,2012,12(27):5230-5233. |
| 辛伐他汀对IFN-γ 诱导的人外周血单个核细胞OX40L 表达的影响 |
| The Effect of Simvastatin on Levels of OX40L in Human Peripheral BloodMononuclear Cells (PBMCs)* |
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| DOI： |
| 中文关键词: 辛伐他汀 OX40 配体 动脉粥样硬化 |
| 英文关键词: Simvastatin OX40 ligand Atherogenesis |
| 基金项目:黑龙江省科技攻关项目（2009-2012），合同编号：GC10C305-2 |
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| 中文摘要: |
| 目的：探讨经IFN-γ 刺激后，人外周血单个核细胞OX40L 表达的变化，以及辛伐他汀对单个核细胞OX40L 表达的影响。方
法：将实验标本随机分为2 组，分别干扰素-γ (IFN-γ）刺激组、辛伐他汀干预组。应用RT-PCR 及Western blotting 技术，观察IFN-γ
诱导的人外周血单个核细胞OX40L 表达情况及辛伐他汀对人单个核细胞OX40L 表达的影响。结果：1.1000 U/ml IFN- 与人单个
核细胞共同培养24h 后，OX40L mRNA 和蛋白水平的表达明显增加。2. 预先给予10 mol/L 的辛伐他汀干预1h 可以明显降低
IFN- 诱导的OX40L 表达。结论：IFN- 可诱导人单个核细胞OX40L 表达。辛伐他汀可以抑制IFN- 诱导人单个核细胞OX40L 表
达的增强，从而可能抑制了OX40L 信号通路介导的与炎症有关的血管损伤，延缓动脉粥样硬化的进程。 |
| 英文摘要: |
| Objective: To observe the effect of simvastatin on serum levels of soluble OX40L (sOX40L) in PBMCs. Methods:
Human peripheral blood mononuclear cells (PBMCs) were isolated, and the effects of simvastatin on OX40L expression were evaluated
by RT-PCR and western blot. Results: OX40L mRNA and protein levels increased after stimulation with 1000 U/ml IFN-where
simvastatin could antagonize this increase at a concentration of 10 mol/L. Conclusions: Simvastatin treatment induced down-regulation
of OX40L mRNA and protein expression in a concentration-dependent manner in human peripheral blood mononuclear cells (PBMCs).
IFN-up-regulates the expression of the mRNA and protein of OX40L and this up-regulation could be reversed in present of simvastatin. |
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