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目的：对比不同剂量rhBMP-2 与多孔CPC 复合后的诱导成骨效应，探讨与多孔CPC 复合后的rhBMP-2 的量效关系。方法：将0.5 mg/ml、1 mg/ml、2 mg/ml、3 mg/ml 4 种不同剂量的rhBMP-2 与多孔CPC 材料复合后，植入36 只小鼠双侧股部肌肉内，分别于术后1 周、2 周及4 周取材，通过大体观察、组织学分析、形态计量学分析、荧光双标测定，观察4 组诱导成骨情况。结果：植入1 周，rhBMP-2 与多孔CPC 材料复合表现出了较明显的剂量依赖性，含有较多rhBMP-2 的材料内诱导形成的骨组织也较多，但骨组织的增加并未随着rhBMP-2 剂量的增加而连续递增，2 mg 组和3 mg 组新生骨组织含量无明显差异(P＞0.05)。植入4 周，新生骨组织向材料内部生长，但此时的新生骨组织面积较2 周增加不显著(P＞0.05)。0.5 mg 组新生骨组织含量仍处于最低水平，而其它三组之间却无明显差异(P＞0.05)。结论：在0.5 mg/ml-2.0 mg/ml 剂量范围，与多孔CPC 复合的rhBMP-2 诱导成骨量与其剂量成正比，最佳剂量为2 mg/ml。

英文摘要:

Objective: To investigate the biological effect of inductive osteogenesis of porous CPC with different dose of rhBMP-2 and the interrelation to the dose of rhBMP-2. Methods: The porous CPC with 0.5 mg/ml, 1 mg/ml, 2 mg/ml, 3 mg/ml rhBMP-2 were respectively implanted into the muscle of 36 mice. The mice were killed at 1, 2 and 4 weeks postoperation. The biological effect of inductive osteogenesis was observed by histology, morphometry and fluorescence. Results: At 1 week, the dose dependent of rhBMP-2 could be observed obviously. The better ossification was observed in the porous CPC with more rhBMP-2. But the newly formed bone did not increase continously with the increase of rhBMP-2. There were no significant difference in newly formed bone between 2 mg/ml group and 3mg/ml group (P＞0.05). At 4 weeks, newly formed bone had grown into the porous CPC. But there were no significant difference in the area of newly formed bone at 4 weeks compared with that at 2 weeks (P＞0.05). The content of new bone in 0.5 mg/ml group was significantly lower than that in other groups. There were no obvious differences in content of new bone among 1 mg/l, 2 mg/l and 3mg/ml groups (P＞0.05). Conclusions: The content of new bone increases with the increasing in the dose of rhBMP-2 in the dose cope of 0.5mg/ml-2.0mg/ml, and the optimal dose of rhBMP-2 is 2.0mg/ml.

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