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目的：探讨DOG-1、CD117、CD34、WT-1在胃肠道间质瘤（GIST）中的表达及临床意义。方法：应用免疫组织化学SP法检测 DOG-1、CD117、CD34、WT-1在39例GIST患者肿瘤组织中的表达。结果：GIST 光镜下主要由梭形细胞和（或）上皮样细胞或多形性细胞混合或单一性组成。DOG-1、CD117、CD34 和WT-1 在GIST 肿瘤组织中阳性表达率分别为92.3%（36/39）、71.7% （28/39）、64.1%（25/39）、23.1%（9/39），四者的阳性表达率在各风险程度组（极低及低度危险性GIST、中度危险性GIST、高度危险性GIST）两两比较中差异均无统计学意义（P＞0.05）。DOG-1 与CD117 相比，在极低及低度危险组中表达有显著差异（P<0.05）， DOG-1与CD34相比，在极低及低度危险组、高度危险组中表达有显著差异（P<0.05）；WT-1与CD117相比，在中度及高度危险组中表达有显著差异（P<0.05），WT-1与CD34相比，在中度危险组中表达有显著差异（P<0.05）。对照组平滑肌瘤、纤维瘤、神经鞘瘤中CD117、DOG-1在GIST中的表达明显高于其他多种梭形细胞的表达（P<0.05）。结论：DOG1 是GIST 较为敏感和特异的标记物，WT-1在极低及低度危险性GIST中有一定的诊断价值，在GIST的诊断中加入两者将使患者更加受益。

英文摘要:

Objective: To investigate the expression of DOG-1,CD117,CD34 and WT-1 in gastrointestinal stromal tumors(GIST) and its diagnostic application. Methods: Immunohistochemical SP technique was used to assess the expression of DOG-1,CD117,CD34 and WT-1 in 39 cases of GIST. Results: All 39 cases of GIST consisted of variable proportion of spindle and epithelioid tumor cells or just one type of the tumor cell. The expression rates of DOG-1, CD117, CD34 and WT-1 were 92.3%（36/39）, 71.7%（28/39）, 64.1% （25/39）, 23.1%（9/39）. The expression rates of DOG-1, CD117, CD34 and WT-1 have no statistical difference in the group of very low and low risk GIST,moderate risk GIST,high risk GIST（P＞0.05）, The sensitivity of DOG-1 detection of very low and low risk GIST were significantly higher than those of CD117（P<0.05）, The sensitivity of DOG-1 detection of very low and low risk GIST, high risk GIST were significantly higher than those of CD34（P<0.05）; The sensitivity ofWT-1 detection of moderate risk and high risk GIST were significantly higher than those of CD117（P<0.05）, The sensitivity of WT-1 detection of moderate risk GIST were significantly higher than those of CD34（P<0.05）. The expression of DOG-1 and CD117 in GIST were much higher than leiomyomas,schwannomas,fibromas （P<0.05）. Conclusion: DOG-1 is a sensitive and specific marker for GIST; WT-1 has some reference value in the diagnosis of very low and low risk GIST; more patients will benefit from the joining of DOG-1 and WT-1.

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