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目的：检测日间和夜间Lewis 肺癌小鼠血清中内血管内皮生长因子（VEGF）水平和肺癌组织中VEGF蛋白表达的差别，探讨肿瘤血管生成的昼夜节律。方法：选择C57BL小鼠30只，制备Lewis肺癌小鼠模型后随机分为日间组（D 组）和夜间组（N 组），在光照- 黑暗条件下饲养建立统一同步化日夜节律。随着成瘤过程，应用ElISA 方法测定两组小鼠模型第0、3、5、7 天血清中 VEGF浓度水平；成瘤后第9天处死小鼠，应用Westeon-blot法检测瘤体中VEGF蛋白的表达，并分别进行相关分析。结果：随着成瘤过程，D 组小鼠血清中VEGF浓度均显著高于N 组（P＜0.05）；D 组小鼠瘤体组织中VEGF蛋白表达灰度值（8.87±1.20）均明显高于N组（6.43±1.35），有统计学意义（P＜0.05）。结论：Lewis小鼠休息期（白天）血清中VEGF浓度及瘤体中VEGF蛋白表达水平均明显高于活动期（夜间），存在着明显的日夜差异，说明肺癌组织的血管生成可能具有一定日夜节律。

英文摘要:

To detect the concentration of vascular endothelial growth factor (VEGF) in serum and the expression of VEGF protein in lung cancer tissue at daytime and nighttime, for investigating the circadian rhythm of tumor angiogenesis. Methods: Lung cancer mouse model made with 30 C57BL mice were divided into daytime group (D group) and nighttime (N group), which were breed and synchronized to same circadian rhythm. To assay the Concentration of VEGF in serum on day 0, 3, 5, 7. To execute mice on day 9 after forming of tumor and to detect the expression of VEGF protein in lung cancer tissue at daytime and nighttime, then to make analysis respectively. Results: The concentration of VEGF in serum and grey scale of VEGF protein in D group were higher than those in N group, and there was significant difference between two groups. Conclusion: The circadian rhythm of tumor angiogenesis could exist in lung cancer tissue.

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