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目的：探讨人白蛋白对体外培养的HK-2 细胞凋亡的作用。方法：本实验研究对象为HK-2 细胞株，将培养的HK-2 细胞与 20g/L的人白蛋白共同孵育0、4、6、8小时后，用Hoechst33258染色检测细胞凋亡。不同浓度(0g/L、5 g/L、10 g/L、20g/L和30g/L)的人白蛋白与体外培养的HK-2 分别共同孵育0、4、6、8 小时后，流式细胞仪检测细胞凋亡。结果：Hoechst33258 染色结果显示：培养基对照组未见明显细胞凋亡；20g/L白蛋白与HK-2细胞共同孵育4、6、8 小时，与对照组比较，均可见HK-2 细胞荧光强度增加，有着典型凋亡形态的细胞增多，且随着人白蛋白与HK-2细胞作用时间的延长，细胞凋亡的程度和数目也增多。流式细胞仪检测结果显示：与对照组比较，HK-2细胞的凋亡率随着人白蛋白与HK-2细胞作用的浓度和时间增加而显著性增高，细胞凋亡率在 8h 组为(9.15±0.15%)，在30g/L组为（9.35±0.46%)，均为最高。结论：人白蛋白以时间和剂量依赖方式诱导肾小管细胞凋亡，以 30g/L作用浓度和8小时作用时间的人白蛋白诱导HK-2细胞凋亡的作用最显著。

英文摘要:

Objective: To investigate the effects of human albumin on the apoptosis of renal tubular epithelial HK-2 cells in vitro．Methods: HK-2 cells were incubated with various concentrations(0g/L, 5g/L, 10 g/L, 20 g/L, 30g/L) of human albumin for 0h, 4h, 6h and 8h. The apoptosis of HK-2 cells were evaluated by Hoechst33258 fluorescence stained cytospins and flow cytometry. Results: No significant apoptosis was observed in the control group treated by normal culture medium, but the fluorescence intensity and number of typical apoptotic cells increased in the cells treated with 20g/L human albumin for 4, 6 and 8 hours, and they increased gradually when the time extended. The flow cytometry results showed that the rates of apoptosis in HK-2 cells rose significantly along with increasing concentrations of human albumin and prolonging of incubation time, which was the highest at concentration of 30g/L and for 8h (respectively 9.15±0.15％ and 9.35±0.46％). Conclusions: Human albumin induced the apoptosis of HK-2 cells in a dose and time-dependent manner. It got the most significant effect when it lasted for 8h at concentration of 30g/L.

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