| 单纯筱王宇尹磊淼徐玉东魏颖冉君刘晓燕刘奇杨永清△.大鼠淋巴细胞培养上清液总IgG 含量ELISA 检测方法的建立[J].现代生物医学进展英文版,2012,12(26):5015-5019. |
| 大鼠淋巴细胞培养上清液总IgG 含量ELISA 检测方法的建立 |
| Establishment of ELISA for Total IgG Content of Rat LymphocyteCulture Supernatant |
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| DOI： |
| 中文关键词: 大鼠淋巴细胞培养上清液 IgG 双抗体夹心ELISA |
| 英文关键词: Cultured rat-lymphocyte supernatant IgG Double-antibody sandwich ELISA |
| 基金项目:国家自然科学基金(81001548；81173341；81173332；30873286)；上海市教委和上海市教育发展基金会“晨光
计划”资助项目(10CG45)；上海市卫生局青年基金(2009Y096)；国家中医药管理局、上海市重点学科建设项目(S30304) |
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| 中文摘要: |
| 目的：建立一种定量测定大鼠淋巴细胞培养上清液中总IgG（免疫球蛋白G）含量的双抗体夹心ELISA（酶联免疫吸附试验）
检测方法。方法：用方阵实验确定包被抗体、检测抗体的最佳工作浓度；绘制标准曲线，确定线性范围；评价标准曲线的可重复性、
精密度和可应用性。结果：包被抗体和检测抗体的最佳效价分别为2μg/ml和1:4000稀释；检测的线性范围为0.25-16ng/ml。经方
法学评价，可重复性和精密度较高，应用性较强。结论：该方法灵敏度高，重复性好，可作为科研过程中检测大鼠淋巴细胞培养上
清液总IgG含量的一种精确、方便、可靠的方法。 |
| 英文摘要: |
| Objective: To establish a quantitative double-antibody sandwich ELISA detecting method for total IgG content of
cultured rat-lymphocyte supernatant. Methods: Detect the optimal concentrations of coated antibody and enzyme-antibody with a matrix
experiment. Construct a calibration curve and determine the linear rang. Evaluation repeatability, precision and applicability of the
calibration curve. Results: The optimal concentration of coated antibody is 2 μg/ml and the optimal dilution ratio of enzyme-antibody is
1:4000. The linear rang of calibration curve is 0.25-16 ng/mL. The repeatability, precision and applicability are excellent. Conclusion:
This ELISA detecting method is convenient and reliable to detect total IgG content of cultured rat-lymphocyte supernatant. |
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