| 赵艳秋1# 张迎媚2# 范圣瑾1 赵艳红1 李晓霞1 张卓1 孙丽丽1.三氧化二砷对急性早幼粒细胞白血病细胞微粒数量及促凝活性的影响[J].现代生物医学进展英文版,2012,12(25):4833-4836. |
| 三氧化二砷对急性早幼粒细胞白血病细胞微粒数量及促凝活性的影响 |
| The Effect of Arsenic Trioxide on Amount and Procoagulant Activity ofMicroparticles Released by Acute Promyelocytic Leukemia Cells* |
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| DOI： |
| 中文关键词: 急性早幼粒细胞白血病 微粒 促凝血活性 组织因子 三氧化二砷 |
| 英文关键词: Acute promyelocytic leukemia Microparticle Procoagulant activity Tissue factor Arsenic trioxide |
| 基金项目:黑龙江省自然科学基金项目（D201010） |
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| 中文摘要: |
| 目的：观察急性早幼粒细胞白血病(APL)细胞来源微粒(APL-MP)的促凝活性、表面组织因子(TF)表达情况、TF 在其促凝活性
中发挥的作用及分化治疗药物三氧化二砷（ATO）对上述指标有何影响。方法：选取3 例初发APL 患者，提取骨髓APL 细胞，3 名
缺铁性贫血患者提取骨髓单个核细胞作为对照。分别用不同浓度ATO 处理APL 细胞24 h、48 h、72 h，收集细胞培养液提取微粒。
采用流式细胞术对微粒进行定量分析并进行微粒表面TF 表达情况检测；利用凝血实验比较不同组细胞释放微粒的促凝血活性；
应用抗TF 抗体抑制微粒促凝血活性实验检测TF 在APL-MP 的促凝血活性中发挥多大作用。结果：1.0 μM 及2.0 μM ATO 能显
著促进APL 细胞释放微粒。与正常骨髓来源单个核细胞释放的微粒相比，骨髓APL-MP 的TF 表达及促凝活性均显著增高，0.5
μM 及1.0 μM ATO 处理可以有效降低APL-MP 的TF 表达及促凝活性，且这一作用呈时间依赖性。各组APL-MP 经抗TF 抗体
孵育后凝血时间显著延长。结论：APL-MP 的TF 表达和促凝学活性均显著增高，并且TF 在APL-MP 的促凝血活性中发挥着重要
作用。ATO 能显著促进APL 细胞释放微粒，低浓度ATO 可以有效降低APL-MP 的TF 表达及促凝血活性。 |
| 英文摘要: |
| Objective: To observe the effect of arsenic trioxide (ATO) on number, tissue factor (TF) expression and procoagulant
activity of microparticles released by acute promyelocytic leukemia (APL) cells, and to identify how much TF plays a role in
procoagulant activity of these microparticles. Methods: APL cells were isolated from bone marrow of 3 newly diagnosed APL patients.
Bone marrow mononuclear cells were collected from 3 patients with iron deficiency anemia and used as control. APL cells were cultured
with medium containing different concentration of ATO for 24 h, 48h or 72h. Microparticles were extracted from the cell culture
medium. The number and TF expression of microparticles were measured by flow cytometry. The procoagulant activities of
microparticles released by different groups of cells were detected by coagulation assays. How much TF plays a role in the procoagulant
activity of APL cells-derived microparticles (APL-MPs) was evaluated by coagulation inhibition assays in which anti-TF antibody was
used to suppress TF procoagulant activity. Results: 1.0 μM and 2.0 μM ATO had a promoting effect on the release of microparticles by
APL cells. Compared with bone marrow mononuclear cells-derived microparticles, the bone marrow APL-MPs showed a higher TF
expression and procoagulant activity. 0.5 μM and 1.0 μM ATO had an inhibitory effect on TF expression and procoagulant activity of
APL-MPs in a time-dependent manner. After preincubation with anti-TF antibody, in most groups, the APL-MPs indicated a significantly
decreased procoagulant activity (prolonged clotting time). Conclusion: Both the TF expression and procoagulant activity of APL-MP
were obviously increased, and TF played an important role in the procoagulant activity of these microparticles. ATO could promote the
release of microparticles by APL cells. Low concentration of ATO could effectively reduce the TF expression and procoagulant activity
of APL-MP. |
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