| 霍莹莹李芸兰成志锋△ 李波王明丽.小檗碱对甲亢性腹泻大鼠结肠及血浆中神经肽Y 含量的影响[J].现代生物医学进展英文版,2012,12(24):4620-4623. |
| 小檗碱对甲亢性腹泻大鼠结肠及血浆中神经肽Y 含量的影响 |
| Investigate the Effect of Berberine on Neuropeptide Y in Rats Colon Tissueand Plasma of Thyroid Hormone-Induced Diarrheic |
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| DOI： |
| 中文关键词: 甲状腺功能亢进 腹泻 小檗碱 神经肽Y |
| 英文关键词: Hyperthyroidism Diarrhea Berberine Neuropeptide Y |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨小檗碱对甲状腺功能亢进（甲亢）性腹泻大鼠结肠及血浆中神经肽Y 的影响，进而研究小檗碱治疗甲亢性腹泻的
机制。方法：制备甲亢性腹泻大鼠模型。应用小檗碱对其干预，采用免疫组化方法测定甲亢性腹泻组、小檗碱治疗剂量组、健康组
大鼠结肠神经肽Y 的定位，采用酶联免疫方法检测各组大鼠血浆中神经肽Y 含量的变化。结果：在结肠组织中，甲亢性腹泻组与
健康对照组比较NPY 表达明显增多，经小檗碱治疗后NPY 表达较甲亢性腹泻组表达减少，接近健康对照组的表达。在血浆中，
NPY 的表达在甲亢性腹泻组与健康对照组中增多，经小檗碱治疗后NPY 表达较甲亢性腹泻组表达减少，接近健康对照组的表
达。结论：甲亢性腹泻大鼠结肠及血浆中NPY 的表达明显增多，小檗碱治疗后NPY 的表达又减少,接近健康对照组。NPY 可能
参与甲亢性腹泻的发生机 |
| 英文摘要: |
| Objective: To investigate the effect of berberine on Neuropeptide Y (NPY) in colon and plasma of thyroid
hormone-induced diarrheic (hyperthyroid diarrhea,HD) rats and to study the mechanism of berberine in the treatment of hyperthyroidism.
Methods: The model of thyroid hormone-induced diarrheic rats were prepared and interfered by berberine. Immunohistochemistry was
used to detect the location of the Neuropeptide Y in colon of hyperthyroid diarrhea group, dose group of berberine and healthy group.
ELISA was used to detect the content of Neuropeptide Y in plasma of each group. Results: NPY expression in colon tissue, hyperthyroid
diarrhea group increased compared with that in the healthy group. NPY expression in berberine treatment group decreased compared with
that in the hyperthyroid diarrhea group, closed to the expression of the healthy control group. In plasma, NPY expression in the
hyperthyroid group and diarrhea in healthy control group increased. NPY expression in berberine treatment group compared with that in
hyperthyroid diarrhea group decreased, and which was close to the expression of the healthy control group. Conclusion: NPY expression
increased significantly in rats colon tissue and plasma of thyroid hormone-induced diarrheic. NPY expression reduced by berberine
treatment, closed to the healthy control group. NPY may be involved in the mechanism of diarrhea in hyperthyroidism. |
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