| 张雷1△ 罗时敏2 张晓坤1 黄国威1 廖一鸣1.富含BCAA 的PN 对肝硬化大鼠肝部分切除术后肝脏NK 的影响[J].现代生物医学进展英文版,2012,12(23):4442-4443. |
| 富含BCAA 的PN 对肝硬化大鼠肝部分切除术后肝脏NK 的影响 |
| Effect of Parenteral Nutrition with Branch-Chain Amino AcidsEnrichment on the Natural Killer Cells in the Liverafter Partial Hepatectomy in Rats with Liver Cirrhosis |
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| DOI： |
| 中文关键词: 肝硬化 肝切除术 支链氨基酸 自然杀伤细胞 |
| 英文关键词: Liver cirrhosis Hepatectomy Branch-chain amino acid Natural killer cells |
| 基金项目:广州市荔湾区科技计划项目（20071209112） |
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| 中文摘要: |
| 目的：探讨富含支链氨基酸的肠外营养对肝硬化大鼠肝部分切除术后肝脏自然杀伤细胞（NK 细胞）的影响。方法：20 只肝硬
化大鼠随机分为肝部分切除术后行8.5%Novamin 的肠外营养5 d 组10 只，肝部分切除术后行10%Hepa 的肠外营养5 d 组10
只。应用流式细胞仪测定大鼠肝脏NK 细胞的百分率；应用4 小时51Cr 释放法测定肝脏NK 细胞的杀伤活性。结果：与8.5%
Novamin 的肠外营养5 d 组比较，10%Hepa 的肠外营养5 d 组肝脏NK 细胞占全部淋巴细胞的百分比和肝脏NK 细胞杀伤活性
明显升高（P<0.05）。结论：富含支链氨基酸的肠外营养可以增加肝脏NK 细胞的百分率和肝脏NK 细胞的杀伤活性。 |
| 英文摘要: |
| Objective: To explore the effect of parenteral nutrition (PN) with branch-chain amino acids enrichment on the natural
killer cells in the liver after partial hepatectomy in rats with liver cirrhosis. Methods: Twenty rats with liver cirrhosis were randomly
divided into post-operation 8.5% Novamin PN 5 day group (n=10) and post-operation 10% Hepa PN 5 day group (n=10). Activity of NK
cells was measured by 4h 51Cr-release assay. The percentages of the NK cells in the livers was examined by flow cytometry. Results:
Compared with the 8.5% Novamine PN 5 day group, the percentages and the activity of NK cells in the livers were significantly increased
in the 10%Hepa PN 5 day group (P<0.05). Conclusion: Parenteral nutrition with branch-chain amino acids enrichment after partial
hepatectomy in rats with liver cirrhosis can ameliorate the percentages and the activity of NK cells in the livers. |
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