| 冯建书付强△ 闫世杰张乐明王江泳周晓彬王海涛.仙灵骨葆对骨质疏松大鼠OPG/RANK/RANKL 表达的影响[J].现代生物医学进展英文版,2012,12(23):4438-4441. |
| 仙灵骨葆对骨质疏松大鼠OPG/RANK/RANKL 表达的影响 |
| Effect of Xianlinggubao on the Expression of OPG/RANK/RANKL inOsteoporotic Rats |
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| DOI： |
| 中文关键词: 骨质疏松 仙灵骨葆 OPG RANK RANKL |
| 英文关键词: Osteoporosis Xianlinggubao OPG RANK RANKL |
| 基金项目:石家庄市科技局（0512038） |
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| 中文摘要: |
| 目的：观察仙灵骨葆治疗骨质疏松模型大鼠后，对大鼠体内OPG/RANKL/RANK 表达的影响。方法：卵巢摘除法建立SD 大
鼠骨质疏松模型，设立假手术组、对照组（单纯去卵巢组）、雌激素组（给予17β- 雌二醇）和治疗组（给予仙灵骨葆）。术后1 周开始
给药，给药12 周后检测各组大鼠股骨骨密度，ELISA 法检测血清中OPG/RANKL 含量，RT-PCR 检测骨组织中OPG/RANK/RANKL
mRNA 表达，免疫组化检测骨组织中RANK 的表达。结果：对照组大鼠骨密度显著低于假手术组；治疗组和雌激素组大鼠OPG
表达显著高于对照组，RANK 及RANKL 的表达显著低于对照组。结论：采用卵巢摘除法成功建立大鼠骨质疏松模型；仙灵骨
葆可促进骨质疏松大鼠OPG 的表达，并抑制RANK 及RANKL 的表达，对骨质疏松模型大鼠有治疗作用。 |
| 英文摘要: |
| Objective：To investigate the impact of Xianlinggubao treatment on OPG/RANKL/RANK expression in osteoporosis
rats. Methods: SD rat model of osteoporosis was established by ovariectomy and divided into four groups: the sham operation group, the
control group (OVX simply), the estrogen group (given 17β-estradiol) and treatment group (given Xianlinggubao). The rats were
administered one week after surgery. After 12 weeks dosing, all rats had their bone mineral density detected. The OPG/RANKL content
in serum were detected by ELISA, and the OPG/RANKL/RANK mRNA expression in bone tissue were detected by RT-PCR, and the
RANK expression in bone tissue were detected by immunohistochemical assay. Results: The bone mineral density in control group was
significantly lower than the sham operation group; The OPG expression in treatment/estrogen group were significantly higher than the
control group; The RANK and RANKL expression in treatment/estrogen group were significantly lower than the control group.
Conclusion: A rat model of osteoporosis was successfully established by the ovariectomy method; Xianlinggubao can promote the
expression of OPG and inhibit the expression of RANK/RANKL in osteoporosis rats. It has a therapeutic role in the rat model of
osteoporosis. |
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