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目的：研究探讨钴- 原卟啉对大鼠脑损伤的保护作用。方法：用钴- 原卟啉灌(50mg/kg)胃处理后，建立液压脑损伤模型，采用免疫组织化学SP 三步法检测大鼠脑损伤区域血红素氧合酶-1（HO-1）的表达情况，用干湿重法测定损伤周边区脑含水量的变化。结果：正常组、假手术组大鼠HO-1 均无表达；模型组脑损伤区域的HO-1 阳性细胞数为（3.45±047）；实验组12h、24h、3d 和7d HO-1 的阳性细胞数分别为：（10.9±1.35）、（10.62±1.88）、（12.4±1.57）和（10.99±2.42）。正常组、假手术组脑损伤周边区脑含水量分别为（78.7±0.4）%和（78.6±0.7）%；模型组脑含水量（89.3±0.3）%；实验组12h、24h、3d 和7d 脑含水量分别为：（83.1± 0.3）%、（83.6±0.6）%、（83.9±0.4）%和（83.2±0.3）%。结论：钴- 原卟啉（CoPP）能够诱导大鼠脑损伤区域HO-1 的表达，减轻脑损伤周边区域的水肿程度，故钴- 原卟啉对大鼠脑损伤具有保护作用。

英文摘要:

Objective: To investigate the protective effect of cobalt protoporphyrin on brain injuries in Rats. Methods: We built hydraumatic brain injuries model after intragastric administration of 50 mg/kg cobalt protoporphyrin. We checked out expression of heme oxygenase-1 of brain injuries zone in Rats with immunohistochemistry SP three-step method, and detected changes of brain water content of brain injury ambitus zone with dry-wet weight regulation. Results: Normal group and sham operation group had no expression of HO-1. HO-1 positive cell count was (3.45±047) in model group, and (10.9±1.35), (10.62±1.88), (12.4±1.57) and (10.99±2.42) at 12 h, 24 h, 3 d and 7 d in experimental group respectively. Brain water content were respectively (78.7±0.4)% and (78.6±0.7)% in normal group and sham operation group, and was (89.3±0.3)% in model group, and respectively(83.1±0.3)%,(83.6±0.6)%,(83.9±0.4)% and (83.2±0.3)% in experimental group at 12 h, 24 h, 3 d and 7 d. Conclusions: Cobalt protoporphyrin can induce the expression of HO-1 of brain injuries zone in rats, and alleviate brain edema of injury ambitus zone. Thus, cobalt protoporphyrin plays a protective role in hydraumatic brain injuries in rats.

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