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目的：比较黑龙江省HIV/AIDS 患者与健康对照者(healthy controls, HCs) 外周血CD4+CD25+FoxP3+ 调节性T 细胞数量、免疫抑制功能的变化，探讨CD4+CD25+FoxP3+ 调节性T 细胞在HIV/AIDS 感染过程中的作用。方法：采用流式细胞仪检测21 例 HIV/AIDS 患者及20 例健康对照组的外周血CD4+CD25+FoxP3+ 调节性T 细胞数量的百分比及绝对数量；采用共同培养方法检测 HIV/AIDS 患者外周血CD4+CD25+FoxP3+ 调节性T 细胞免疫抑制功能的变化；实时荧光定量聚合酶链反应(RT-FQ-PCR) 检测 HIV/AIDS 患者外周血CD4+CD25+FoxP3+ 调节性T 细胞中FoxP3mRNA 的表达。结果：黑龙江省HIV/AIDS 患者外周血 CD4+CD25+FoxP3+ 调节性T 细胞比率明显高于HCs (P<0.01)，而CD4+CD25+FoxP3+ 调节性T 细胞的绝对计数显著下降，且与 CD4+T 细胞绝对计数成反比；混合淋巴细胞共同培养结果显示，HIV/AIDS 患者外周血CD4+CD25+FoxP3+ 调节性T 细胞的抑制功能无明显变化；HIV/AIDS 患者外周血CD4+CD25+FoxP3+ 调节性T 细胞的FoxP3 mRNA 相对表达量无显著变化。结论：黑龙江省 HIV/AIDS 患者CD4+CD25+FoxP3+ 调节性T 细胞的数量变化与病情相关。

英文摘要:

Objective: To analyze the number, suppressive function of CD4+CD25+FoxP3+ regulatory T cells in peripheral blood from patients with HIV/AIDS and healthy controls and to vestigate the role of CD4+CD25+FoxP3+ regulatory T cells in the process of HIV/AIDS. Methods: Flow cytometry was used to analyze the frequency of CD4+CD25+FoxP3+ regulatory T cells from 21 patients with HIV/AIDS and 20 healthy controls; Functional characterization of Tregs from the peripheral blood mononuclear cells (PBMC) of patients and healthy controls were analyzed by suppression of proliferation by co-cultured effector CD4+CD25+ T cells. Foxp3 message (mRNA) expression level was assessed by quantitative real-time polymerase chain reaction. Results: A significantly increased relative frequency of CD4+CD25+FoxP3+ regulatory T cells within the CD4 compartment of HIV/AIDS patients compared to that of healthy controls (P<0.0001) was observed. Additionally, there was a significant negative correlation between the frequency of CD4+CD25+FoxP3+ regulatory T cells and CD4 count, despite HIV/AIDS patients having lower absolute counts of CD4+CD25+FoxP3+ regulatory T cells. On the other hand, HIV/AIDS-derived CD4+CD25+FoxP3+ T cells and that from healthy individuals exhibited equal FOXP3-expression of mRNA and their ability of suppressing the proliferation and cytokine secretion of CD4+ effector T cells was unimpaired in HIV/AIDS patients. Conclusions: These results suggest that the frequency of CD4+CD25+FoxP3+ regulatory T cells in HIV/AIDS patients of Heilongjiang Province significantly correated with disease progression.

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