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目的：观察艾灸不同穴位对束缚应激模型大鼠行为学改变的影响，进一步探讨艾灸对束缚应激所致心理行为改变的作用规律。方法：将35 只雄性Wistar 大鼠按分层随机法分为正常组、束缚应激模型组、艾灸百会（GV20）组、艾灸关元（CV4）组、艾灸足三里（ST36）组，每组各7 只。除正常组外，余各组均采用自制布袋束缚大鼠30 min，每日1 次，共20 次，制备束缚应激大鼠模型，于造模第2 天各治疗组捆绑固定后给予艾炷灸3 壮，正常组及模型组捆绑束缚20 min，隔日一次，共10 次。于造模前、造模后，治疗 5 次、10 次分别采用高架十字迷宫检测各组大鼠开放臂进入次数比例(OE% )及开放臂停留时间比例(OT% )的变化。结果：①与造模前比较，艾灸百会（GV20）组大鼠造模后、治疗10 次后OE%均降低明显，治疗5 次后OT%升高明显，均有显著性差异（P<0. 05），艾灸关元（CV4）组大鼠造模后OT%降低明显，有显著性差异（P<0.05），艾灸足三里（ST36）组大鼠造模后OE%和OT%、治疗 10 次后OE%均明显降低，均有显著性差异（分别为P<0.05，P<0.05，P<0.01）；与造模刚结束比较，艾灸百会（GV20）组大鼠治疗5 次后OE%与OT%均升高明显，均有显著性差异（P<0.05），艾灸关元（CV4）组大鼠治疗10 次后OT%升高明显，有显著性差异（P<0.01），艾灸足三里（ST36）组大鼠治疗5 次后OE%、治疗10 次后OT%均明显升高，有显著性差异（分别为P<0.01，P<0.05）；与治疗5 次比较，艾灸百会（GV20）组、足三里（ST36）组大鼠治疗10 次后OE%明显降低，艾灸关元（CV4）组大鼠治疗10 次后OE% 和OT%升高明显，均有显著性差异（P<0.05）。②与艾灸百会（GV20）组比较，艾灸关元（CV4）组与艾灸足三里（ST36）组大鼠在治疗5 次、10 次后OE %和OT%呈不同程度的升高或降低趋势，未见显著性差异（P>0.05）。结论：艾灸不同穴位可一定程度改善慢性束缚应激所致大鼠焦虑心理行为的变化；关元（CV4）、百会（GV20）、足三里（ST36）三穴均表现出一定抗焦虑效应，百会（GV20）穴在治疗早期效果显著，足三里（ST36）穴的疗效肯定，而关元（CV4）穴在长期治疗中效应更为稳定、明显，具有相对特异性。

英文摘要:

Objective: To observe the influence of moxibustion at different acupoints on behavioral changes caused by restraint stress and further discussion on regulation rule of moxibustion for psychological and behavioral changes caused by restraint stress. Methods: According to the principle of stratified randomization the 35 male Wistar rats were divided into the normal group, the model group, Baihui group, Guanyuan group and Zusanli group, 7 rats in each group. Except the normal group, the rest groups were tied by self-made bags for 30min each day, totally lasting 20 days to make the restraint stress model. From the 2nd day after model made, the treatment groups were treated by moxibustion for 3 cones on each acupoint, once every other day, a total of 10 times. All groups were respectively tested into the elevated plus-maze to detect the changes of the proportion of the number of entry into the elevated plus-maze open arms (OE%) and that of Residence time at the open arms (OT%) at four time points, including before and after modeling, after the 5th treatment and 10th treatment. Results: Compared with that in the groups before modeling, the OE % of the rats in Baihui group decreased significantly after modeling、after the 10th treatment and the OT% of the rats in Baihui group increased significantly after the 5th treatment (P<0.05), after modeling the OT % of the rats in Guanyuan group decreased significantly (P<0.05), after modeling the OE% and OT % of the rats, after the 10th treatment the OE % of the rats in Zusanli group all decreased significantly (P<0.05,P<0.05,P<0.01); Compared with that in the groups after modeling, after the 5th treatment the OE% and OT% of the rats in Baihui group both increased significantly (P< 0.05), after the 10th treatment the OT % of the rats in Guanyuan group increased significantly (P<0.01), the OE% of therats in Zusanli group after the 5th treatment and the OT % of the rats in Zusanli group after the 10th treatment both increased significantly (P<0.01，P<0.05); Compared with that in the groups after the 5th treatment, The OE % of rats in Baihui group and Zusanli group both decreased significantly after the 10th treatment (P<0.05), the OE% and OT% of rats in Guanyuan group increased significantly after the 10th treatment (P<0.05). Compared to that in Baihui group, the OE % and OT % of the rats in Guanyuan and Zusanli group had different changes，but no significant difference (P>0.05). Conclusions: Moxibustion at different acupoints could improve anxiety and changes in emotion and behavior induced by restraint stress; Guanyuan, Baihui and Zusanli all having some anxiolytic effect, Baihui having better effect in the early period of stress, Zusanli having a reliable effect, but Guanyuan having a much more stable and obvious effect after long-term treatment, which had relative specificity.

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