| 冯晶晶1 雷炜1△ 姚如永2 赵园园1 阎超1.靶向survivin 的siRNA 联合5-FU 转染抑制肝细胞株HepG2 增殖的研究[J].现代生物医学进展英文版,2012,12(18):3446-3449. |
| 靶向survivin 的siRNA 联合5-FU 转染抑制肝细胞株HepG2 增殖的研究 |
| Study on the Antiproliferation Effect of Targeting of Survivin siRNATransfection Combined with 5-FU on Liver Cell Line HepG2 |
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| DOI： |
| 中文关键词: SiRNA survivin 肝癌 5-FU |
| 英文关键词: siRNA Survivin Liver neoplasms 5-FU |
| 基金项目: |
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| 中文摘要: |
| 目的：研究靶向survivin 的（小分子干扰RNA）siRNA 和（氟尿嘧啶）5-FU 联用对肝癌细胞HepG2 的增殖抑制及凋亡的影
响。方法：将HepG2 细胞分为空白对照组、阴性对照组、5-FU 处理组、siRNA 转染组、5-FU+siRNA 转染组。转染采用脂质体法。
RT-PCR 法检测HepG2 细胞survivin mRNA 转录水平；MTT 法检测靶向survivin 的siRNA 和5-FU 对HepG2 细胞增殖的抑制作
用；流式细胞术检测HepG2 细胞凋亡情况。结果：空白对照组、阴性对照组、5-FU 处理组survivin mRNA 表达无明显变化(P>0.
05)，siRNA 转染组、5-FU+siRNA 转染组survivin mRNA 表达明显下降(F=280.326，q=4.72~7.34，P<0.05)。5-FU+siRNA 转染组增
殖抑制率为51.58％±1.35％，与其它各组相比抑制率明显增高(F=280.326，q=5.27~9.84，P<0.05)。5-FU+siRNA 组与其它各组相
比细胞凋亡率明显增高(F=13568.68，q=110.47～327.16，P＜0.01)。结论：将靶向survivin 的siRNA 和5-FU 联合应用可以显著抑
制肝癌细胞survivin 基因表达，并协同抑制HepG2 细胞增殖，共同发挥诱导细胞凋亡作用。 |
| 英文摘要: |
| Objective: To investigate the anti-proliferation effect of (small interference RNA) siRNA against survivin combined
with(fluorouracil) 5-FU on human liver cancer cell line HepG2. Methods: HepG2 cells cultures were divided into five groups: blank control
group, negative control group, 5-FUgroup, siRNA-survivin group, siRNA-survivin+5-FU group. Lipofectamine TM 2000 was used to
transfect HepG2 cell. The expression of survivin mRNA was detected by RT-PCR. Inhibition rate of each group on HepG2 was assayed
by MTT method, and apoptosis was analyzed by flow cytometry respectively. Results: The experiment of RT-PCR showed that the expression
of survivin mRNA in siRNA-survivin group and siRNA-survivin+5-FU group were lower than that in other groups(P<0.05), but
its levels in blank control group, negative control group and 5-FU group had no significant change (P>0.05). The MTT assay indicated
that the antiproliferation rate of siRNA-survivin+5-FU group was 51.38%±1.35. The antiproliferation rate of this group was higher than
that in any other groups(P<0.05). Flow cytometry results indicated that the apoptosis rate of siRNA-survivin+5-FU group was also higher
than any other groups (P<0.05). Conclusion: siRNA against survivin combined with 5-FU can specifically suppress its expression, inhibit
the proliferation of HepG2 and induce apoptosis in common. |
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