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目的：观察穿心莲内酯(Andrographolide, AD)对人非小细胞肺癌细胞系H3255 细胞的生长抑制作用，并研究其对肿瘤生长相关标记物血管内皮生长因子（vascular endothelial growth factor, VEGF）、转化生长因子（transforming growth factor，TGF）β1 的表达以及蛋白激酶C（protein kinase C，PKC）的活性有无影响。方法：体外培养H3255 细胞，分别用1.0、2.5、5.0 μmol/L 的AD 在处理细胞24h。MTT 法检测细胞的增殖，比色法检测乳酸脱氢酶（lactate dehydrogenase，LDH）的释放情况。ELISA 检测DNA 片段化情况以及VEGF 和TGF-β1 的产生；无机磷法检测Na+-K+-ATP 酶活性以及磷基转移法测得PKC 活性。结果：AD 处理能呈剂量依赖性方式降低H3255 细胞的活性以及Na+-K+-ATP 酶活性（P<0.05），同时也能促进LDH 的释放和DNA 片段的形成，并减少肺癌细胞VEGF 和TGFβ1 的水平和PKC 的磷酸化。结论：AD 对肺癌细胞具有一定的抑制作用，有望成为一种潜在的肿瘤治疗药物。

英文摘要:

Objective: To observe the growth suppression effect of Andrographolide (AD) on non-small cell lung cancer cell line H3255, and to study the expression of tumor growth associated biomarkers such as vascular endothelial growth factor (VEGF)，transforming growth factor β1 (TGF-β1) and the activity of protein kinase C (PKC). Methods：H3255 cells were cultured in vitro, and incubated with 1.0, 2.5 or 5.0 μmol/L AD for 24h. MTT assay was performed to examine cell viability, LDH activity was determined by colorimetric methods. DNA fragmentation, expression of VEGF and TGF-β1 were detected by ELISA. The ATPase activity and PKC activity were tested by inorganic phosphorus detection method and phosphoryl abevacuation, repectively. Results：AD treatments concentration-dependently decreased cell viability, and lowered Na+-K+-ATPase activity (P<0.05). This compound also increased DNA fragmentation and LDH releasing. It also reduced VEGF and TGF-β1 levels, and inhibited protein kinase C activity in H3255 cells (P<0.05). Conclusion： AD can inhibit lung cancer cells growth, and thus it may be a potent anti-lung cancer agent.

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