| 许勇刚翁明哲张金彦徐军明△ 彭志海.单人建立大鼠原位肝移植模型的手术体会[J].现代生物医学进展英文版,2012,12(16):3026-3028. |
| 单人建立大鼠原位肝移植模型的手术体会 |
| Technique of Orthotopic Liver Transplantation in Rats Operated by OnePerson without Surgical Microscope |
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| DOI： |
| 中文关键词: 肝移植 大鼠 动物模型 |
| 英文关键词: Liver transplantation Rat Animal model |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨单人建立稳定大鼠原位肝移植模型手术中的难点及对策。方法：单人及双人裸视下采用改良二袖套法制备大鼠
原位肝移植模型各50 例。结果：单人及双人组供体手术时间、无肝期、受体手术手术时间分别为：（39.16±2.89）min 和（38.36±
3.04）min、（19.92±1.36）min 和（19.70±1.40）min、（61.98±3.46）min 和（58.65±3.94）min；单人及双人组手术成功率、1 周存活率、
一月存活率分别为：94.0 %（47/50）和92.0 %（46/50）、86.0 %（43/50）和88.0 %（44/50）、86 %（43/50）和84 %（42/50）。结论：通过改
进手术方法，简化操作，单人即可建立稳定的大鼠原位肝移植模型。 |
| 英文摘要: |
| Objective: To investigate the difficulties and countermeasures of the surgical technique in establishing a stable rat
model of orthotopic liver transplantation. Methods: The model was established with improved two cuff technique. One hundred cases of
rat orthotopic liver transplantation were performed by single person and double persons without surgical microscope. Fifty cases were
operated in each group. Results: In single operator group and double operators group, donor operation time, anhepatic time and receptor
operation time were(39.16±2.89)min vs (38.36±3.04) min, (19.92±1.36) min vs (19.70±1.40) min, (61.98±3.46) min vs (58.65±
3.94) min; The successful operation rate, one week survival rate and one month survival rates in single operator group and double operators
group were 94.0 % (47/50) vs 92.0 % (46/50), 86.0 % (43/50) vs 88.0%( 44/50), 86 % (43/50) vs 84 % (42/50) respectively. Conclusion:
Through improving technique, it is successful to establish a stable model of rat orthotopic liver transplantation by single operator without
surgical microscope. |
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