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目的：探讨原发性肝癌患者血清中淋巴细胞亚群中Th1/Th2 的变化, 为分析肝癌的发生发展状症和临床治疗提供免疫学指标。方法：应用放射免疫分析及酶联免疫分析法（ELISA），测定46 例肝癌患者，及43 例正常对照组进行比较。以IL-2、INF-γ 和 TNF-α 水平代表Th1 型细胞因子，以IL-4，IL-6、IL-8、IL-10 的水平代表Th2 型细胞因子。结果：肝癌患者IL-2、TNF-γ、IL-6 的水平明显低正常对照组,P<0.01。IL-4、IL-8、IL-10、TNF-α 的水平明显高于正常对照组，P<0.01。结论：肝癌患者体内存在Th1/Th2 细胞因子失衡，其中Th1 亚群功能抑制，Th2 亚群功能亢进，其与肿瘤在宿主体内生长密切相关。通过纠正这些免疫失调将成为肝癌治疗的重要手段。

英文摘要:

Objective: To study the changes of Th1/Th2 subsets of CD4 + cells in peripheral blood of patients with primary hepatocellular carcinoma (PHC) to analyze the occurrence and progression of primary PHC and provide a immunological data for clinical treatment. Methods: RIA and ELISA were used to detect the level of serum IL-2, IL-4, IL-6, IL-8, IL-10 TNF-γ and TNF-а in 46 patens with PHC 43 normal serum as control. IL-2, TNF-γ and TNFα were used to represent cytokines of Th1 type, and IL-4, IL-6, IL-8 and IL-10 to represent cytokines of Th2 type. Results: The level of IL-2, TNF-γ, IL-6 in PHC group were significantly lower than those in the normal group (P<0.01), but the levels of IL-4, IL-8, IL-10 and TNFα were significantly higher in patients with PHC than those in normal control group (P<0.01). Conclusions: The levels of IL-2，IL-4, IL-8 and IL-10 were relatively sensitive for diagnosis of PHC. In the peripheral blood of the patients, there seems to be disregulation in the function of Th1/Th2 subsets of CD4 + T cells with enhancement of Th2 function and a malfunction in Th1 cells. These changes are closely related to tumor progresses in the host. Correcting this immune malfunction may become an important method in hepetocallular carcinoma.

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