| 任庆先1 王建村2 王莉3 高殿帅4 虞正权1.人脑胶质瘤细胞中GDNF 基因启动子1 区甲基化状态的研究[J].现代生物医学进展英文版,2012,12(15):2819-2821. |
| 人脑胶质瘤细胞中GDNF 基因启动子1 区甲基化状态的研究 |
| Study of the Methylation Status of Promoter 1 Region of GDNF Gene inHuman Glioma Cells |
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| DOI： |
| 中文关键词: 胶质瘤细胞 角质细胞源性神经营养因子 启动子 甲基化 |
| 英文关键词: Glioma cell GDNF Promoter Methylation |
| 基金项目:江苏省高校自然科学基金（11KJB180012）；青年科学基金项目（81101899） |
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| 中文摘要: |
| 目的：观察GDNF 启动子1 区在人脑胶质瘤细胞中的甲基化修饰状态，以期探讨其对于GDNF 在胶质瘤中高表达的影响。
方法：基因测序检测10 例胶质瘤与5 例正常脑组织中GDNF 基因序列，比较其基因是否有突变发生；重亚硫酸盐修饰后基因测
序检测20 例胶质瘤（10 例低级别和10 例高级别）与5 例正常脑组织中GDNF 启动子1 区甲基化修饰状态。结果：GDNF 启动子
1 区基因在胶质瘤中没有发生突变；GDNF 启动子1 区甲基化修饰在正常脑组织、低级别、高级别中发生率分别为72.25%、
86.25%、86.75%。在胶质瘤中的甲基化修饰水平比正常脑组织明显增高（P＜0.05），而高低级别之间无显著性差异。结论：在胶质
瘤细胞中，GDNF 启动子1 区发生了高甲基化修饰，这种修饰很可能会影响GDNF 基因的表达。 |
| 英文摘要: |
| Objective: To observe the methylation status of GDNF promoter 1 region in human glioma, in order to explore the
effect of methylation on the expression of GDNF in glioma. Methods : Genesequencing detected the GDNF gene order in 10 cases of
glioma and 5 normal brain tissues, to compare whether their genetic mutations have occurred; the methylation modified status of GDNF
promoter 1 region in 20 cases of glioma (10 cases of low-level and 10 cases of high-level) and 5 cases of normal brain tissues were
detected through genesequencing after bisulfite modification. Results : The mutations of GDNF gene in promoter 1 region were not
observed in glioma; the methylation of GDNF gene in promoter 1 region in normal brain tissues, low-level and high-level glioma were
72.25%, 86.25%, 86.75% respectively. The methylation level in glioma was significantly higher when compared to normal brain tissues
(P＜0.05); while there was no significant differences in high-and low-level tissues. Conclusions: Hypermethylation occuerred in GDNF
promoter 1 region might influence the expression of GDNF in glioma cell. |
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