| 杨平1 寇明文1 赵戈1 卢鹏1 宋振顺2△.L- 精氨酸和雨蛙素诱导急性胰腺炎模型的对比研究[J].现代生物医学进展英文版,2012,12(15):2810-2813. |
| L- 精氨酸和雨蛙素诱导急性胰腺炎模型的对比研究 |
| Comparison of the Model of Acute Pancreatitis Induced by L-arginine andCerulein |
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| DOI： |
| 中文关键词: 急性胰腺炎 L- 精氨酸 雨蛙素 模型 |
| 英文关键词: Acute pancreatitis L-arginine Cearulein Model |
| 基金项目: |
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| 中文摘要: |
| 目的：研究L- 精氨酸和雨蛙素分别诱导SD 大鼠急性胰腺炎（AP）模型的差异，为进一步研究急性胰腺炎提供可靠模型。方
法：L- 精氨酸采用3 次腹腔注射，间隔1 h，雨蛙素采用7 次腹腔注射，间隔1 h 诱导急性胰腺炎模型。碘- 淀粉比色法检测血清
淀粉酶水平，血清脂肪酶测定试剂盒检测脂肪酶活性，胰腺组织切片观察组织的破坏情况，TUNEL 法检测腺泡细胞凋亡。结果：
①L- 精氨酸诱导的大鼠模型血清淀粉酶和脂肪酶水平在诱导成功后6 h 即显著升高，蛙皮素诱导的大鼠模型在12 h 显著升高，
与正常对照组比较均有统计学差异(P<0.05)，提示急性胰腺炎建模成功。②L- 精氨酸诱导的模型中胰腺组织结构破坏，有大片出
血坏死灶、大量炎细胞浸润；而蛙皮素诱导的模型组织腺泡、间质水肿，炎性细胞浸润，少量散在出血坏死灶，血管变化常不明显，
渗液清亮。结论：L- 精氨酸和雨蛙素均能诱导SD 大鼠急性胰腺炎模型，L- 精氨酸诱导重症急性胰腺炎，雨蛙素诱导轻型急性胰
腺炎，是研究急性胰腺炎的良好模型。 |
| 英文摘要: |
| Objective: To investigate the difference of the models of acute pancreatitis(AP) induced by L-arginine and cerulein, so
as to provide reliable models for further study of the acute pancreatitis. Method: Acute pancreatitis was induced in Sprague-Dawley rats
either by giving three intraperitoneal injections of L-arginine, each at concentrations of 200 mg/100g body weight, with an interval of 1 h,
or by giving seven intraperitoneal injections of cerulein, each at concentrations of 40 μg/kg body weight, with an interval of 1 h. Severity
of AP was assessed by analysis of serum amylase and lipase activity. Samples of pancreas were taken for measuring hematoxylin-eosin
staining. Apoptosis in pancreatic acinar cell was determined with terminal deoxynucleotidyl transferase-mediated deoxyuridine
triphosphate nick-end labeling technique (TUNEL). Result : ①Serum amylase and lipase activity has significantly increased at 6 h
timepoint in the model of AP induced by L-arginine, and more higher with the extension of time. It has significantly increased at 12 h
timepoint in the model induced by cerulein. Both have significantly difference(P<0.05). ②The administration of L-arginine caused severe
necrotizing pancreatitis, including large hemorrhage focal necrosis, and obvious inflammatory infiltration. However, there were edema of
acinus and interstitial, a small amount of inflammatory cell infiltration in the model, which induced by cerulein. Conclusion : Acute
pancreatitis were induced by L-arginine and cerulein. L-arginine could induce severely, and cerulein induce mildly. There both are
feasible models of acute pancreatits for further research. |
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