| 卢鹏 冯薇 李霄 杨平 寇明文 胡延忠 宋振顺.肝细胞癌患者外周血HSF1 基因表达的检测及应用价值[J].现代生物医学进展英文版,2012,12(14):2716-2719. |
| 肝细胞癌患者外周血HSF1 基因表达的检测及应用价值 |
| Development and Application of Real-time Quantitative Polymerase ChainReaction for the Detection of Human Hepatocellular Carcinoma RelatedNovel Gene HSF1 Expression |
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| DOI： |
| 中文关键词: 肝细胞癌 外周血 热休克转录因子1 实时定量PCR 诊断 |
| 英文关键词: Hepatocellular carcinoma Serum HSF1 Real-time PCR Diagnosis |
| 基金项目:国家自然科学基金（30971508） |
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| 中文摘要: |
| 目的：建立检测HSF1 mRNA 的real-time PCR 的方法，了解肝细胞癌患者外周血中HSF1 的表达水平及其与各临床病理特
征之间的关系。方法：利用real-time PCR 的方法检测20 例肝细胞癌患者及20 例正常人群外周血中HSF1 mRNA 的表达量。结
果：肝细胞癌患者外周血中的HSF1 mRNA 表达量显著高于正常人群（P＜0.05）；肝细胞癌患者外周血中HSF1 mRNA 的表达水
平在不同性别、肿瘤大小、门静脉侵犯情况、HbsAg 水平及AFP 水平的患者中的差异无统计学意义（P＞0.05）；在不同病理分化程
度、TNM 分期的患者中的差异有统计学意义（P＜0.05）。结论：real-time PCR 技术可以成功检测外周血中HSF1 mRNA 的表达量，
HSF1 可能与肝细胞癌的发生发展密切相关。 |
| 英文摘要: |
| Objective: To establish a quantitative real-time PCR method for detection of HSF1 gene expression level in human
hepatocellular carcinoma and to investigate the relationship between HSF1 mRNA expression level and the clinicopathological
characters. Methods: Real-time PCR was employed to determine the expression level of HSF1 mRNA in peripheral blood of 20
specimens of human hepatocellular carcinoma patients and normal human. Results: Comparing with normal human, HSF1 present higher
expressions in human hepatocellular carcinoma patients (P<0.05). There is no statistical differences of HSF1 mRNA expression level
between the group with different sexes, tumour diameters, portal involvement levels, HBsAg levels and AFP levels (P＞0.05）.
There is
statistical differences of HSF1 mRNA expression level between the group with different differentiations and TNM stages (P＞0.05）.
Conclusion: The established quantitative real-time PCR method can successfully detect the expression level of HSF1 mRNA, which may
be closely related to the occurrence and development of human hepatocellular carcinoma. |
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