| 李争鸣周秀萍林梅李秀萍.口服卡介菌诱导免疫耐受的CD4+CD25+调节性T 细胞机制研究[J].现代生物医学进展英文版,2012,12(12):2278-2281. |
| 口服卡介菌诱导免疫耐受的CD4+CD25+调节性T 细胞机制研究 |
| Studies on CD4+CD25+ T Regulatory Cells in Immune ToleranceInduced by Oral BCG* |
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| DOI： |
| 中文关键词: 卡介菌 免疫耐受 实验性变态反应性脑脊髓炎 CD4+CD25+T 淋巴细胞 |
| 英文关键词: BCG Immune tolerance EAE CD4+CD25+T regulatory cell |
| 基金项目:湖南省教育厅课题资助项目（06C069） |
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| 中文摘要: |
| 目的：研究口服卡介菌诱导免疫耐受对CD4+CD25+ 调节性T 细胞的影响。方法：采用口服MPB 制备EAE 大鼠模型，随机分
为BCG 组（0.5mg/kg）和EAE 模型组（PBS），每组各15 只，连续经口灌服给药14d，同时选取15 只健康大鼠作为对照组。分别于
免疫后15d、27d 流式细胞术检测外周血、胸腺及脾脏中CD4+CD25+T 淋巴细胞百分率，ELISA 检测血清IL-6、TGF-β、IgE、IgG 含
量。结果：与EAE 模型组相比，免疫后BCG 组大鼠外周血、胸腺及脾脏中CD4+CD25+T 淋巴细胞百分率增加，血清IL-6、TGF-β
含量上升，血清IgE、IgG 抗体水平下降。结论：口服BCG 通过上调淋巴器官中CD4+CD25+T 淋巴细胞比例，抑制效应性T 细胞活
性，发挥免疫耐受作用。 |
| 英文摘要: |
| Objective: To investigate the influence of oral BCG on CD4+ CD25+ T regulatory cells in inducing immune tolerance.
Methods: The EAE rats, induced by immunization with oral MBP, were randomly divided into BCG group (0.5mg/kg) and EAE group
(PBS), with 15 rats per group, and 15 normal rats were used as control group. On 15d and 27d after treatment, CD4+ CD25+ T regulatory
cells from peripheral blood, thymus and spleen were respectively determined by flow cytometry, and levels of serum IL-6, TGF-β, IgE
and IgG were measured by ELISA. Results: Compared with EAE group, CD4+ CD25+ T regulatory cells from peripheral blood, thymus
and spleen, and levels of IL-6 and TGF-β increased in BCG group after treatment, but serum IgE and IgG levels decreased. Conclusion:
Oral BCG could induce immune tolerance by up-regulating the percentage of CD4+ CD25+ T regulatory cells, inhibiting effector T cell
function. |
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