| 赵喜聪1 朱斌1 段妍1 孙聪1 冯源1 王国轩2 金岩1 李德华1△.两种不同诱导液对人牙周膜干细胞膜片性能的影响[J].现代生物医学进展英文版,2012,12(12):2220-2223. |
| 两种不同诱导液对人牙周膜干细胞膜片性能的影响 |
| Effects of Two Different Inducing Liquids on PDLSCs Sheet |
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| DOI： |
| 中文关键词: 牙周膜干细胞 牙周膜干细胞膜片 DAC 诱导液 成骨诱导液 |
| 英文关键词: Periodontal ligament stem cells (PDLSCs) Periodontal ligament stem cells (PDLSCs) sheet DAC induction medium Osteogenic induction medium |
| 基金项目:国家重点基础发展规划项目（973 项目）（2010CB944803） |
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| 中文摘要: |
| 目的：对比分析发育期根端复合体（Dental Apical Complex，DAC）诱导液和成骨诱导液对人牙周膜干细胞（Periodontal ligament
Stem Cells，PDLSCs）膜片形成和分化能力的影响。方法：收集临床十名12-16 岁的青少年因正畸需要拔除的健康、无龋的新鲜
牙（前磨牙），刮取根面残留牙周膜进行牙周膜干细胞的分离培养，制备成牙周膜干细胞膜片，分别采用DAC 诱导液和成骨诱导
液对膜片进行诱导，对比分析两种诱导液对牙周膜干细胞膜片的影响。结果：DAC 诱导液处理过的PDLSCs 细胞膜片，细胞形态
接近牙源性间充质细胞，DAC 诱导液提供了良好的成牙骨质- 牙周样结构诱导环境，可促进PDLSCs 膜片向功能性成牙骨质细
胞谱系分化，而成骨诱导液提供了良好的成骨诱导环境，可促进PDLSCs 膜片向功能性成骨细胞谱系分化。结论：DAC 诱导液较
之于成骨诱导液更利于PDLSCs 细胞膜片的成牙周样分化，利于牙周缺损修复和局部再生。 |
| 英文摘要: |
| Objective: To investigate the effects of dental apical complex (DAC) inducing liquid and osteogenesis inducing liquid
on PDLSCs (Periodontal ligament Stem Cells)sheet. Methods: PDL tissues were separated from the surface of the mid-third of root and
culture PDLSCs and PDLSCs sheet. PDLSCs sheet were induced with DAC inducing liquid and osteogenesis inducing liquid. Results:
PDLSCs sheet induced by DAC inducing liquid which provides microenvironment for induction to cementum-PDL like tissue showed the
morphous of dental mesenchyme-like tissue.Osteogenesis inducing liquid provides osteogenetic microenvironment which promotes
osteogensis induction. Conclusion: Compared with osteogenesis inducing liquid, DAC inducing liquid is beneficial to PDLSCs sheet
differentiation to PDL-like tissue to repair periodontal defeats and regeneration locally. |
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