| 何娟1 陈琳1，2 张伟光1 姜山1 李凤兰1 李晖1△.miR-30b 的组织特异性研究[J].现代生物医学进展英文版,2012,12(12):2209-2211. |
| miR-30b 的组织特异性研究 |
| A Research on the Tissue-Specific of miR-30b |
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| DOI： |
| 中文关键词: 微小RNA miR-30b 组织特异性 实时荧光定量PCR |
| 英文关键词: MicroRNA MiR-30b Tissue-Specific Expression Real-Time Quantitative PCR |
| 基金项目:国家自然科学基金资助（30771863；81172616）；哈尔滨市科技局优秀学科带头人基金（RC2008XK004039) |
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| 中文摘要: |
| 目的：研究miR-30b 的组织特异性，检测其在心，肝，脑，肾，脾和骨骼肌中的表达情况。方法：选取C57BL/6J 雄性小鼠6
只，用real-time PCR 方法检测小鼠心，肝，脑，肾，脾和骨骼肌中miR-30b 的表达量。结果：miR-30b 在小鼠肝脏中表达量最低，与
肝相比，在心，脑，肾，脾和骨骼肌中的相对表达量分别为13.13±0.899，9.497±0.717，4.478±1.031，6.751±0.596，2.538±0.79。且与肝
相比均具有统计学意义(P<0.05)。结论：miR-30b 在各组织中的表达存在差异，在心脏中的表达量最高，提示miR-30b 可能与心
脏的发生发展密切相关，为深入探索miR-30b 的功能奠定了基础。 |
| 英文摘要: |
| Objective: To investgate the tissue-specific of miR-30b and to detect the expression of miR-30b in hearts, livers,
brains, kidneys, spleens and skeleton muscles of mice. Methods: The Real-time Quantitative PCR method were used to detect the
expression levels of miR-30b in hearts, livers, brains, kidneys, spleens and skeleton muscles of 6 male C57BL/6J mice. Results: The
expression level of miR-30b in livers of mice was the lowest. Compared the expression level in livers, the relative expression levels of
miR-30b in hearts, brains, kidneys, spleens and skeletal muscles were respectively 13.13±0.899, 9.497±0.717, 4.478±1.031, 6.751±0.596,
2.538±0.79. They all had significant differences compared with the expression level of livers (P<0.05). Conclusion: The expression levels
of miR-30b were different in various tissues, which had the highest expression in hearts. This suggests that miRA-30b may be closely
related to the growing and development of hearts. It lays a foundation for exploring the function of miR-30b in depth. |
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