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目的：探讨在父系HBeAg 阳性的流产胚胎中，乙型肝炎病毒在绒毛中的表达。方法：募集仅父系感染乙型肝炎病毒组合，即母HBsAg（-）且父HBsAg（+）流产胚胎。按以下组合将入选对象分为4 组：组1 为父HBeAg（+）母HBsAb（+）；组2 为父HBeAg （+）母HBsAb（-）;组3 为父HBeAg（-）母HBsAb（+）; 组4 为父HBeAg（-）母HBsAb（-），采用酶联免疫吸附实验（ELISA）对胎儿父、母亲血清进行乙肝抗原、抗体检测，并使用荧光定量PCR 法对胚胎绒毛进行HBV DNA 检测。结果：父系感染乙型肝炎病毒的 142 例胚胎中，仅在父系HBeAg 阳性组别（1、2 组）84 例胚胎中发现3 例绒毛HBV-DNA 升高，阳性率为3.57%。其中父HBeAg （+）母HBsAb（-）组合中2 例，父HBeAg（+）母HBsAb（+）组合中1 例。父系HBeAg 均阳性, 母系HBsAb 阳性与阴性组间子代绒毛HBV-DNA 升高率差异无显著性（P>0.05）。结论：HBeAg 阳性父亲可能更容易导致乙肝父婴垂直传播。

英文摘要:

Objective: To investigate hepatitis B virus (HBV-DNA) expression in villi from paternal HBeAg positive early abortion embryo. Methods: Early abortion embryos, whose paternal serum HBsAg positive and maternal serum HBsAg negative, were collected. They were divided into G1 paternal HBeAg (+)and maternal HBsAb (+), G2 paternal HBeAg (+) and maternal HBsAb (-), G3 paternal HBeAg (-) and maternal HBsAb (+), and G4 maternal HBsAb (-) and paternal HbeAg (-). parents serum HBV antigen and antibody measurement was performed by ELISA, HBV-DNA expression in villi was measured by fluori-metric quantilative PCR. Results: Among 142 villi from paternal HBsAg positive early abortion embryo, 3 of 84 villi from paternal HBeAg positive early abortion embryo were detected for HBV-DNA expression. The positive rate was 3.57%. 2 cases in G2 paternal HBeAg (+) and maternal HBsAb (-), and 1 case in G1 paternal HBeAg (+) and maternal HBsAb (+). In groups whose paternal HBeAg were positive (G1 and G2), there was no significant difference for villi HBV-DNA expressing rate (P>0.05). Conclusion: Hepatitis B virus might more likely transmit vertically to early embryo villi in paternal HBeAg positive cases.

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