张蕴霞1 姜君仪2 刘俊江3 戴琪1 牛建清1 王健1.多个生物标志物预测宫颈上皮内瘤和宫颈癌的风险[J].现代生物医学进展英文版,2012,12(10):1930-1933. |
多个生物标志物预测宫颈上皮内瘤和宫颈癌的风险 |
Predicting Progression of Cervical Intraepithelial Neoplasia, and Prognosis ofCervical Cancer with a Panel of Biomarkers Tested in Multivariate Modeling |
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DOI: |
中文关键词: 宫颈癌 生物标志物 宫颈上皮内瘤 |
英文关键词: Cervical cancer Biomarkers CIN |
基金项目: |
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中文摘要: |
目的:研究生物蛋白指标是否可以作为宫颈癌和高分级宫颈上皮内瘤样病变(CIN)的肿瘤标志物。方法:采用免疫组织化学
方法检测包括宫颈内皮瘤和宫颈癌在内的292 例标本中9 个蛋白指标的表达,包括E- 钙粘素(E-cadherin),细胞外信号调节激酶
-1(ERK-1),基质金属蛋白酶-2(MMP-2),nm23-H1,核因子NF-κB,p16INK4A,存活素(survivin),人端粒酶逆转录酶(hTERT),
血管内皮生长因子(VEGF-C),然后采用统计方法进行分析,构建变量模型,计算反映指标,与靶受体反应的特征曲线进行比较,
确定上述蛋白标记物在预测宫颈癌存活率以及高分级CIN 的作用。结果:所有个标记物中,nm23-H1 及p16 INK4a 对宫颈癌的
生存率的单变量分析有明显的意义, 在对所有标记物做细致详尽的分析后发现,其中3 个标记物(E- 钙粘素、VEGF-C、survivin)可
对高分级的CIN 进行预测。宫颈癌的生存率的有效的预测因子中,只有nm23-H1 可以作为有效的预测因素。结论:联合检测多个
生物蛋白,如E- 钙粘素、VEGF-C、survivin 指标可以有效的预测高危CIN 的恶变风险,同时nm23-H1 则可以为宫颈癌的预后提
供参考。 |
英文摘要: |
Objective: To investigate if the 9 markers would be independent predictors of intermediate end point markers in
cervical carcinogenesis. Methods: 9 markers(E-cadherin, ERK-1, MMP-2, nm23-H1, NF-κB, p16INK4A, survivin, hTERT, VEGF-C)
were Immunohistochemical (IHC) stained, then multivariate models were constructed to test predictive power of the markers for 2
outcomes. Results: In all the markers, the most powerful predictors of CIN2 (and above) lesions were E- cadherin, VEGF-C, survivin.
The nm23-H1 was significant predictor of CC survival in univariate analysis. Conclusions: Combining multiple markers can accurate
detection of high-grade CIN in a clinical setting, and the markers could be the indicators for the survival of cervical cancer. |
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