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目的：了解我院5 年来耐甲氧西林金黄色葡萄球菌( MRSA)对万古霉素敏感性的变化，为临床治疗和控制该类感染提供依据方法：采用以肉汤稀释法测定近5 年耐甲氧西林金黄色葡萄球菌（MRSA）临床分离株对万古霉素的最低抑菌浓度(MIC)，按NCCLS/ CLSI2010 年标准判定结果，WHONET5.5 软件进行数据分析。结果：MRSA 检出率从2005 年的19.23 %上升到2009 年的42 .14 % ，万古霉素的M IC 几何均数从0.54 μg/ml 上升到1.21 μg/ml，未检出万古霉素、替考拉宁耐药株，但2007 年有2 株，2008 年、2009 年各有1 株菌株对替考拉宁呈中介，抑菌圈直径依次为12 mm、12 mm、13 mm、12 mm，MIC 值均为16.0 μg/ml。结论： MRSA 检出率逐年上升，MRSA 对万古霉素MIC 值有升高的趋势，加强金黄色葡萄球菌对万古霉素敏感性的监测非常必要。

英文摘要:

Objective: To review and analyze the evolution of the MICs of vancomycin in methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in our hospital during an five - year period to guide rational use of antimicrobial agents. Methods: The MICs of vancomycin were detected by broth macrodilution method on methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from clinical specimens dring last 5 years ,etermination of the results observed the standard of CLSI version 2010, and drug sensitivity analysis adopted WHONET5. 5. Results: MRSA detection rate from 19.23 % in 2005 rose to 42.14 % in 2009, MICs geometri mean of vancomycin increased from 0.54 μg/ml to 1.21 μg/ml, No isolate was found drug resistanceto vancomycin and teicoplanin，Four strain of MRSA from 2007 to 2009 was intermediate to teicoplanin,The diameters (mm) of the inhibition zone were 12.0,12.0,13.0,12.0; The M ICs of vancomycin to 4 isolates were 4μg/ml. Conclusion: MRSA microorganisms are increasing from 2007 to 2009, MICs of vancomycin in MRSA indicated a trend of increasing; It is important to monitor the antimicrobial susceptibility of Staphylococcus aureus to vancomycin.

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