| 倪伊婷1 马伟康1 齐先福1 张连祺1 王君德2 辛毅1△.东亚钳蝎粗毒蛋白质热稳定性的研究[J].现代生物医学进展英文版,2012,12(10):1846-1848. |
| 东亚钳蝎粗毒蛋白质热稳定性的研究 |
| Thermal Stablility Of Scorpion Venom ProteinsFrom Bathus Martiensii (BMSV) |
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| DOI： |
| 中文关键词: 蝎毒 热稳定性 聚丙烯酰胺凝胶电泳 半数致死量(LD50) |
| 英文关键词: BMSV Thermal stability SDS-PAGE Median lethal dose(LD50) |
| 基金项目: |
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| 中文摘要: |
| 目的：通过蝎毒热溶解性和毒性实验，探索蝎毒蛋白稳定性与分子量间的关系。方法：对蝎毒粗毒煮沸后的可溶成分进行急
性毒力实验和SDS-PAGE 电泳分析。结果：蝎毒粗毒加热处理组的LD50 为16.228mg/kg, 粗毒对照组的LD50 为3.66mg/kg，显示蝎
毒经加热后毒力明显降低，但仍具有一定的毒性。SDS-PAGE 结果显示，经加热处理的粗蝎毒之沉淀主要为大分子蛋白质，在可
溶成分中含有小分子肽类。结论：蝎毒的大分子蛋白质热稳定性差，而小分子多肽部分耐热性强，而且蝎毒的小分子多肽组分具
有相对稳定的毒性。 |
| 英文摘要: |
| Objective: To investigate the relationship between the thermal stablility and protein molecular weight of scorpion
venom from Bathus martiensii (BMSV) by detecting the solubility and toxicity after boilling. Methods: Boiled and non-boiled BMSV
were centrifuged, and the supernatants were detected by SDS-PAGE analysis and toxicity detection. Results: Supernatant from boiled
BMSV showed higher LD50 than that from non-bioled BMSV, and most proteins with high molecular weight were preciapteted and
exhibated low toxicity. Supernatant from boiled samples maintained certain toxicity. Conclusion: The proteins with hjgh molecular
weight from BMSV had low thermal stablility while the small peptides from BMSV had high thermal stablility. |
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