| 罗光伟1 徐玮2 吴霜3 万军1
黄燕玲1 杨硕1 张念1 徐清1 孙洁民1 肖宏4△.地塞米松对哮喘豚鼠血清白细胞介素-4 变化及肺组织形态的影响[J].现代生物医学进展英文版,2012,12(10):1828-1830. |
| 地塞米松对哮喘豚鼠血清白细胞介素-4 变化及肺组织形态的影响 |
| Effects of Dexamethasone on Serum IL-4 and the Pathological Changes ofLung Tissue of Guinea Pig with Asthma |
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| DOI： |
| 中文关键词: 地塞米松 哮喘 豚鼠 IIL-4 肺组织病理 |
| 英文关键词: Examethasone Asthma Guinea pig IL-4 Lung morphology |
| 基金项目:武汉市卫生局资助课题（武卫[2001]235 号） |
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| 中文摘要: |
| 目的：观察地塞米松对哮喘豚鼠血清白细胞介素－4（IL-4）及肺组织形态的影响，探讨糖皮质激素治疗哮喘的机制。方法：
30 只健康雄性Hartley 系豚鼠随机分为空白对照组，病理模型组，地塞米松组。用卵白蛋白（OVA）复制哮喘豚鼠模型。采用流式细
胞术分析CD4+interleukin-4(IL-4)细胞占CD4+T 细胞的比例，光镜检查肺组织形态变化。结果：病理模型组外周血中IL-4 明显高
于空白对照组（P＜0.05），地塞米松组外周血中IL-4 较病理模型组明显降低（P＜0.05），且地塞米松组支气管上皮损伤,粘液腺增
生等病理改变较病理模型组明显改善。结论：地塞米松治疗哮喘的作用与抑制Th2 的活化，改善支气管上皮损伤, 粘液腺增生等
病理改变有关。 |
| 英文摘要: |
| Objective: To observe the influence of dexamethasone on serum IL-4 and the pathological changes of lung tissue of
guinea pig with asthma. and to explore the mechanism of dexamethasone in the treatment of asthma. Methods: 30 healthy male Hartley
guinea pigs were divided into three groups randomly and equally: control group, model group, dexamethasone treatment group.The
model guinea pigs of asthma was induced by intraperitoneal injection of ovalbumin.The level of CD4+ IL-4 was tested by flow cytometer
and analyzed statistically. Lung histological changes were examined by HE stains and under microscopy. Results: IL-4 increased
obviously in model group compared with control group （P＜0.05）. The levels of IL-4 in serum of dexamethasone treatment groups were
significantly lower than those of model group (P<0.05). The bronchial tube epider-mis damage and the mucous membrane gland
proliferation were obviously improved in dexamethasone treatment group compared with model group. Conclusion: Dexamethasone
could relieving asthma according to inhibiting the activation of Th2 and improveing the lung tectology changes. |
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