| 刘扬1 史明1 杜可军2 张鹏3 赵钢1.HLRP 蛋白在大鼠脑中的定位及LPS 对其表达的影响[J].现代生物医学进展英文版,2012,12(10):1801-1804. |
| HLRP 蛋白在大鼠脑中的定位及LPS 对其表达的影响 |
| Distribution of HLRP in Rat Brain and Effects of LPS Lateral VentricleInjection on HLRP Expression |
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| DOI： |
| 中文关键词: 脂多糖 神经元 HLRP 基因 小胶质细胞 星形胶质细胞 |
| 英文关键词: LPS Neuron HLRP Astrocyte Microglia |
| 基金项目:国家自然科学基金项目(31170801) |
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| 中文摘要: |
| 目的：研究HLRP 分子在大鼠脑中的细胞定位和表达特点，观察LPS 刺激对动物脑HLRP 表达的影响。方法：对原代培养的
大鼠神经元、星形胶质细胞、小胶质细胞和大鼠脑组织冰冻切片分别进行免疫荧光染色，观察HLRP 的细胞定位和表达特点；给
大鼠侧脑室注射LPS，提脑组织蛋白，进行Western blot 检测，半定量分析LPS 刺激后，大鼠脑HLRP 的表达变化。结果：①HLRP
选择性表达于部分神经元的细胞核中，正常的星形胶质细胞和小胶质细胞不表达HLRP。②从嗅脑到脑干各节段，HLRP 在大鼠
脑组织中均匀分布，未发现HLRP 阳性神经元聚集的现象。③侧脑室注射LPS 1 天以后，HLRP 表达明显升高(P<0.05)。结论：大鼠
脑中正常表达HLRP，侧脑室注射LPS 能刺激HLRP 表达。 |
| 英文摘要: |
| Objective: To investigate the location of HLRP on different cells in rat brain, and the effect of LPS lateral ventricle injection
on HLRP expression. Methods: Neurons, astrocytes, and microglias were cultured seperately, and identified with anti-Tuj1, anti-GFAP,
anti-OX42 IgG respectively. Then the three types of cells were co-labled with anti-HLRP IgG to investigate the location of HLRP.
Discontinous frozen rat brain slices were gathered from olfactory brain to brainstem, then incubated with anti-HLRP IgG to observe the
expression of HLRP. LPS solution was injected into rat lateral ventricle, and the expression of HLRP was analyzed with Westernblot
method. Results: ① There was no HLRP expression in the neucleus of a certain subset of neurons, and astrocytes and microglias in
normal conditions.②HLRP expression in rat brain showed no difference from olfactory brain to brainstem, and no HLRP positive neuron
clusters were observed. ③ After the injection LPS solution to rat lateral ventricle, HLRP expression increased obviously 1d later (P<0.
05). Conclusion: HLRP expressed at normal rat neurons, and HLRP expression increased after injection LPS solution to rat lateral
ventricel. |
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