| 沈红石任传路陈海飞李征洋唐杰庆吴天勤△.T-bet、GATA-3 与T 细胞亚群在再障中的相关性研究[J].现代生物医学进展英文版,2012,12(9):. |
| T-bet、GATA-3 与T 细胞亚群在再障中的相关性研究 |
| Expression of T-bet, GATA-3 mRNA and T cell subsetsin in peripheralblood of patients with aplastic anemia |
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| DOI： |
| 中文关键词: 再生障碍性贫血 T-bet GATA-3 |
| 英文关键词: Aplastic anemia T-bet GATA-3 |
| 基金项目:全军医药卫生科研项目（07M018） |
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| 中文摘要: |
| 目的：探讨外周血中T-bet 和GATA-3 mRNA 的表达在再生障碍性贫血中的发病机制及意义。方法：入选27 例再障患者，其
中重型再障15 例，轻型再障l2 例，25 例健康体检者为对照组。采用流式细胞术检测参试者外周血Thl 和Th2 细胞，RT-PCR 检测
外周血单核细胞中转录因子T-bet 和GATA-3 mRNA 的表达。结果：与健康对照组相比，再障患者血浆T-bet mRNA 与Th1 细胞
比例显著升高(P<0.01)，GATA-3 mRNA 与Th2 细胞明显降低(P<0.05、P<0.01)。与轻型再障患者相比，重型再障患者血浆转录因子
T-bet mRNA 及Th1 细胞比例均明显升高(P<0.01)，GATA-3 mRNA 水平与Th2 细胞比例也显著下降(P<0.05、P<0.01)。结论：T-bet
与GATA-3 异常表达可增强Th1 细胞功能，抑制Th2 细胞功能，导致患者免疫功能异常，最终引起再障发生、发展。 |
| 英文摘要: |
| Objective: To investigate the pathogenic role of T-bet and GATA-3 mRNA expression in aplastic anemia(AA). Methods:
27 patients with AA including 15 cases of severe AA and 12 cases of mediate AA, and 25 healthy people (control group) were selected.
Flow cytometry was used to detect the expression of Thl and Th2 in peripheral blood, and RT-PCR to quantify T-bet and GATA-3 mRNA.
Results: Compared with the control group, the expression of Thl and T-bet mRNA in the AA group increased significantly (P<0.01),
while the expression Th2 and GATA-3 mRNA decreased (P<0.05, P<0.01). Conclusion: Abnormal expression of T-bet and GATA-3
plays an important role in the development and progression of AA, with the possible mechanism of enhancing Thl cells and inhibting Th2
cells. |
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