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目的：研究ICAM-1 基因K469E 位点、MCP-1A2518G 位点基因多态性及sICAM-1、MCP-1 在血清中表达水平与EV71 手足口病的关系，探讨EV71 型手足口病的遗传易感因素。方法：运用限制性片段长度多态性－聚合酶链反应(PCR-RFLP)检测急性期 EV71 感染阳性的手足口病患儿和正常儿童中ICAM-1K469E 位点及MCP-1A2518G 位点碱基变异情况，同时采用双夹心抗体法 (ELISA)检测血清sICAM-l 和MCP-1 水平。结果：EV71 手足口病组患儿血清中sICAM-l 和MCP-1 水平均显著高于正常对照组(P 均<0.01)。EV71 手足口病组ICAM-1K469E 位点中，A 等位基因的频率显著低于对照组（x2=6.897，P<0.01）。EV71 手足口病组患儿MCP-1 基因型分布、等位基因频率与对照组比较均无统计学意义(P>0.05）。结论：sICAM-1 表达水平和其基因K469E 位点多态性与EV71 手足口病有关，A 等位基因可降低EV71 手足口病发生率。MCP-1 表达水平与EV71 手足口病感染有关，但 MCP-1A-2518G 位点基因多态性与EV71 手足口病感染无关。

英文摘要:

Objective: To investigate the relationship between ICAM-1, MCP-1 plasma level and gene polymorphisms with the susceptibility of enterovirus71 infection in hand, foot and mouth disease. Methods: By reverse transcription polymerase chain reaction (RT-PCR), EV71 specific primer was used to detect enterovirus71 in the serum of Hand, Foot and Mouth Disease (HFMD) children. PCR-RFLP techniques were performed to determine the genotypes of 1CAM-1 K469Eand MCP-1 A-2518G gene in HFMD group and in control group. The plasma levels of sICAM-1 and MCP-1 were detected by enzyme-linked immunosorbent assay. Results: Compared with that in the control cases, the plasma levels of sICAM-1 and MCP-1 of the HFMD patients were significantly increased (P< 0.01). In HFMD group, A allele of ICAM-1 K469E were less common than that in control group (x2=6.897, P< 0.01). There were no significant differences in the distribution of frequency of MCP-1A-2518G genotype and allele frequency between the HFMD group and the control group (P> 0.05). Conclusion: Plasma level of sICAM-1 expression and the polymorphism of ICAM-1 K469E single nucleotide had relationship with EV71 infection of HFMD. A allele of ICAM-1K469E may reduce the incidence of enterovirus71 infection in HFMD. Plasma level of MCP-1 expression may had relationship with EV71 infection, butthere was no association between A／G polymorphism in MCP-1A-2518G gene and EV71 infection.

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