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目的：观察支气管哮喘病儿外周血单个核细胞（PBMC）来源树突状细胞（DC）功能变化。方法：以18 例健康儿童为对照，选择16 例支气管哮喘发作期病儿为研究对象，分离PBMC 并经rhGM-CSF 诱生成熟DC。采用流式细胞仪（FACS）检测DC 表面共刺激分子CD80（B7-1）、CD86(B7-2)和CD83 的表达率；ELISA 法检测培养上清液中IL-10 和IL-12 的变化。结果：①哮喘组DC 表面CD86 的表达率明显高于健康对照组（t=2.27，P<0.05），CD80、CD83 的表达率与健康对照组比较均无显著性差异（t =1.17，1.34； P>0.05）。②哮喘组DC 分泌IL-10、IL-12 水平均明显低于健康对照组(t'=3.31，3.39；P<0.01)。③哮喘组DC 分泌IL-10 与IL-12 成正相关（r=0.740，P<0.01），而健康对照组IL-10 与IL-12 无相关性（r=0.232，P>0.05）。结论：支气管哮喘病儿DC 存在功能缺陷，主要表现在CD86 表达升高、IL-10、IL-12 分泌减少。

英文摘要:

Objective: To investigate the function changes dendritic cells (DCs) from peripheral blood mononuclear cells (PBMC) in bronchial asthmatic children. Methods: PBMC-derived immature DCs(iDCs) were obtained from peripherial blood samples in 16 asthmatic patients and in 18 healthy children. iDCs were stimulated with rhTNF-α as mature DCs. The expression of the co-stimulatory molecules CD80(B7-1), CD86(B7-2) and CD83 were detected by fluorescent activated cell sorter and the levels of IL-10 and IL-12 in the supernatant were determined by enzyme-linked immunosobent assay. Results: ①The expression of CD86 on DCs was significantly higher than that of healthy control group (t=2.27, P < 0.05). There was no significant difference in the expression of CD80, CD83 between the two groups(t=1.17, 1.34; P > 0.05). ②The level of IL-10 and IL-12 produced from DCs of asthmatic group were significantly lower than that of healthy control group (t'=3.31, 3.39; P < 0.01).③IL-10 had postively relationship with IL-12 in asthma group (r=0.740, P<0.01), while there was no correlation in healthy control group (r=0.232, P > 0.05). Conclusion: DCs dysfunction existed in asthmatic children. It mainly manifested in the increase of the expression of CD86 and the decrease of the secretion of IL-10 and IL-12.

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