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目的：观察柴胡渗湿汤对哮喘大鼠血清中IL-5 及IL-13 含量的影响，探讨柴胡渗湿汤治疗哮喘的作用机制。方法：将84 只雄性Wi star 大鼠按随机数字表法分为正常对照组、模型对照组、地塞米松组、定喘汤组、柴胡渗湿汤低剂量组、柴胡渗湿汤中剂量组、柴胡渗湿汤高剂量组，每组12 只，采用卵蛋白制作大鼠哮喘模型,予相应药物干预。用酶联免疫吸附试验(ELISA)法检测各组大鼠血清中IL-5 及IL-13 水平。实验数据采用SPSS11.5 统计软件进行分析。结果：实验后各组死亡率比较均无差异，P>0.05；模型对照组与正常对照组血清中IL-5 及IL-13 含量水平有显著差异，表明大鼠哮喘模型存在着血清IL-5 及IL-13 含量异常增高的病理状态；与模型对照组相比较，各治疗组血清中IL-5 及IL-13 的含量明显降低，其中尤以地塞米松组、柴胡渗湿汤中剂量组和柴胡渗湿汤高剂量组的作用更明显。结论：柴胡渗湿汤治疗哮喘的作用机制与降低IL-5 及IL-13 的水平有关；上述作用具有一定的量效关系，但并不因给药剂量的增加而增加。

英文摘要:

Objective: To investigate the effects of radix bupleuri dampness-percolating decoction on the IL-5 and IL-13 in the rat asthma model serum and its function mechanism of asthma treatment. Methods: Eighty-four healthy male Wistar rats were divided by random number method into normal control group, model control group, dexamethasone group, dingchuan decoction group, low dosage radix bupleuri dampness-percolating decoction group, medium dosage radix bupleuri dampness-percolating decoction group and high dosage radix bupleuri dampness-percolating decoction group, with 12 in each group. Then the model of rat asthma was made with ovalbumin and intervened by relevant medicine. The IL-5 and IL-13 in rat serum from each group were tested by enzyme-linked immunosorbent assay (ELISA); experimental data were analyzed by SPSS11.5 statistical software. Results: Mortality ratio of each group after experiment had no difference, P>0. 05. There existed significant difference between the model control group and the normal control group in terms of serum IL-5 and IL-13 content, which indicated a pathological state that serum IL-5 and IL-13 in rat asthma model abnormally increased. Compared with model control group, IL-5 and IL-13 in treatment groups' serum obviously decreased, especially to the dexamethasone group, medium dosage radix bupleuri dampness-percolating decoction group and high dosage radix bupleuri dampness-percolating decoction group. Conclusion: The function mechanism of radix bupleuri dampness-percolating decoction for asthma treatment is related to reduction of the IL-5 and IL-13 level. A certain dose-response relationship existed in the said effect, but the effect does not become stronger with higher dosage.

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